Título:	Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis : more is not necessarily better
Autor(es):	Silva, Calliandra Maria de Souza Hurtado, Fabián Andrés Tavares, Aldo Henrique Fonseca Pacheco Oliveira Júnior, Getúlio Pereira de Raiol, Tainá Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia
ORCID:	https://orcid.org/0000-0002-9064-0735 https://orcid.org/0000-0003-0579-3380 https://orcid.org/0000-0001-6940-401X https://orcid.org/0000-0003-2242-4798 https://orcid.org/0000-0001-5615-1746 https://orcid.org/0000-0001-8656-5835 https://orcid.org/0000-0003-3323-5300 https://orcid.org/0000-0001-8160-7784 https://orcid.org/0000-0003-1403-9363
Assunto:	Paracoccidioides brasiliensis Células dendríticas Resistência Autofagia Fungos
Data de publicação:	2020
Editora:	MDPI
Referência:	DE-SOUZA-SILVA, Calliandra M. et al. Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis : more is not necessarily better. Journal of Fungi, v. 6, n. 4, 311, 2020. DOI: https://doi.org/10.3390/jof6040311. Disponível em: https://www.mdpi.com/2309-608X/6/4/311. Acesso em: 19 maio 2021.
Abstract:	Most people infected with the fungus Paracoccidioides spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to P. brasiliensis to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with P. brasiliensis, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model.
Licença:	Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI:	https://doi.org/10.3390/jof6040311
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