Título:	Zikv strains elicit different inflammatory and anti-viral responses in microglia cells
Autor(es):	Oliveira, Fernanda Bellaniza Caminha de Freire, Vanessa Paola Alves Sampaio de Sá Coelho, Sharton Vinicius Antunes Meuren, Lana Monteiro Palmeira, Julys da Fonseca Cardoso, Ana Luísa Neves, Francisco de Assis Rocha Ribeiro, Bergmann Morais Argañaraz, Gustavo Adolfo Arruda, Luciana Barros de Argañaraz, Enrique Roberto
ORCID:	https://orcid.org/0000-0003-0794-7160 https://orcid.org/0000-0001-5145-4561 https://orcid.org/0000-0002-7011-7197 https://orcid.org/0000-0002-4359-7594
Afiliação do autor:	University of Brasília, Faculty of Health Science, Department of Pharmacy, Laboratory of Molecular Neurovirology University of Brasília, Faculty of Health Science, Department of Pharmacy, Laboratory of Molecular Neurovirology Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goes, Departamento de Virologia, Laboratório de Genética e Imunologia das Infecções Virais Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goes, Departamento de Virologia, Laboratório de Genética e Imunologia das Infecções Virais University of Brasília, Faculty of Health Science, Department of Pharmacy, Laboratory of Molecular Neurovirology University of Coimbra, Centre for Neuroscience and Cell Biology University of Brasília, Faculty of Health Science, Laboratory of Molecular Pharmacology University of Brasilia, Cell Biology Department, Laboratory of Bacuolovirus University of Brasília, Faculty of Health Science, Department of Pharmacy, Laboratory of Molecular Neurovirology Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goes, Departamento de Virologia, Laboratório de Genética e Imunologia das Infecções Virais University of Brasília, Faculty of Health Science, Department of Pharmacy, Laboratory of Molecular Neurovirology
Assunto:	Vírus Zika Infecções por vírus Zika Micróglia Inflamação
Data de publicação:	26-Mai-2023
Editora:	MDPI AG
Referência:	OLIVEIRA, Fernanda Bellaniza Caminha de et al. Zikv strains elicit different inflammatory and anti-viral responses in microglia cells. Viruses, [S. l.], v. 15, n. 6, p. 1250, 2023. DOI: https://doi.org/10.3390/v15061250. Disponível em: https://www.mdpi.com/1999-4915/15/6/1250. Acesso em: 26 ago. 2026.
Abstract:	In recent years, the Zika Virus (ZIKV) has caused pandemic outbreaks associated with a high rate of congenital ZIKV syndrome (CZS). Although all strains associated with worldwide outbreaks derive from the Asian lineage, the reasons for their enhanced spread and severity are not fully understood. In this study, we conducted a comparative analysis of miRNAs (miRNA-155/146a/124) and their cellular targets (SOCS1/3, SHP1, TRAF6, IRAK1), as well as pro- and anti-inflammatory and anti-viral cytokines (IL-6, TNF-α, IFN-γ, IL-10, and IFN-β) and peroxisome proliferator-activated receptor γ (PPAR-γ) expression in BV2 microglia cells infected with ZIKV strains derived from African and Asian lineages (ZIKVMR766 and ZIKVPE243). BV2 cells were susceptible to both ZIKV strains, and showed discrete levels of viral replication, with delayed release of viral particles without inducing significant cytopathogenic effects. However, the ZIKVMR766 strain showed higher infectivity and replicative capacity, inducing a higher expression of microglial activation markers than the ZIKVPE243 strain. Moreover, infection with the ZIKVMR766 strain promoted both a higher inflammatory response and a lower expression of anti-viral factors compared to the ZIKVPE243 strain. Remarkably, the ZIKKPE243 strain induced significantly higher levels of the anti-inflammatory nuclear receptor—PPAR-γ. These findings improve our understanding of ZIKV-mediated modulation of inflammatory and anti-viral innate immune responses and open a new avenue to explore underlining mechanisms involved in the pathogenesis of ZIKV-associated diseases.
Unidade Acadêmica:	Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR) Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Licença:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/v15061250
Aparece nas coleções:	Artigos publicados em periódicos e afins

Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.