Titre:	Produção de proteases por fungos endofíticos : uma revisão sistemática
Auteur(s):	Bezerra, Victor Hugo Souto
metadata.dc.contributor.email:	victorhugosbe_@hotmail.com
Orientador(es)::	Souza, Paula Monteiro de
Assunto::	Proteases Produção Purificação Fungos endofíticos Proteases fibrinolíticas
Date de publication:	17-mar-2022
Data de defesa::	29-nov-2021
Référence bibliographique:	BEZERRA, Victor Hugo Souto. Produção de proteases por fungos endofíticos: uma revisão sistemática. 2021. 87 f., il. Dissertação (Mestrado em Ciências Farmacêuticas) — Universidade de Brasília, Brasília, 2021.
Abstract:	Proteases are produced by almost all living organisms and are responsible for catalyzing the breaking of peptide bonds of proteins into amino acids and peptides. In industries, proteases are used in the production of food, pharmaceutical ingredients, detergents, animal feed, chemicals, and photography. Endophytic fungi are defined as microorganisms that inhabit the internal tissues of plants in a symbiotic way without causing any apparent harm. In recent years, endophytic fungi have shown themselves to be a source of great potential to produce secondary metabolites with promising applications. The purpose of this systematic review was to identify the available literature of production, purification, and characterization of proteases by endophytic fungi. There are few complete studies that entirely exhibit production, characterization, and purification of proteases from endophytic fungi. This study followed the Preferred Reporting Items for Systematic Reviews (PRISMA), and the search was conducted on five databases: PubMed, PMC, Science Direct, Scopus Articles and Web of Science up until September 10th, 2021, with no time or language restrictions. The methodology of the selected studies was evaluated using GRADE. Protease production, optimization, purification, and characterization were the main evaluated outcomes. Of the 6028 initially gathered studies, 15 met the inclusion criteria after a two-step selection process. Only two studies optimized the medium composition using statistical design for enhanced protease production and two reported the total process of purification and characterization of the enzyme. The genus Penicillium was the most cited among the eleven different genera of endophytic fungi evaluated in the selected articles, followed by Aspergillus, Alternaria and Xylaria. Six studies proved the ability of some endophytic fungi to produce fibrinolytic proteases, demonstrating that endophytic fungi can be exploited as producers of fibrinolytic proteases for further production of agents used in thrombolytic therapy. However, further characterization and physicochemical studies are required to evaluate the real potential of endophytic fungi as sources of industrial enzymes.
metadata.dc.description.unidade:	Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
Description:	Dissertação (mestrado) — Universidade de Brasília, Faculdade de Ciências da Saúde, Brasília, 2021.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Ciências Farmacêuticas
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Collection(s) :	Teses, dissertações e produtos pós-doutorado

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