Titre:	Desenvolvimento e avaliações de formulações tópicas contendo derivados de Eugenia dysenterica DC
Auteur(s):	Silva, Sandra Márcia Mazutti da
Orientador(es)::	Batista, Pérola de Oliveira Magalhães Dias
Assunto::	Cagaita Medicamentos fitoterápicos Agentes antibacterianos Cicatrização de feridas Anti-inflamatórios Staphylococcus aureus
Date de publication:	13-aoû-2021
Data de defesa::	27-mar-2018
Référence bibliographique:	SILVA, Sandra Márcia Mazutti da. Desenvolvimento e avaliações de formulações tópicas contendo derivados de Eugenia dysenterica DC. 2018. 181 f., il. Tese (Doutorado em Ciências da Saúde)—Universidade de Brasília, Brasília, 2018.
Abstract:	This study has as objective to analyze the quality control stage of the drug and its plant derivatives, namely the aqueous extract (EBA) and the essential oil (oEd) of the leaves of Eugenia dysenterica Mart. DC., and to develop formulations containing these plant derivatives for treatment of skin lesions. The in vitro antimicrobial activity of EBA and oEd was evaluated against the Staphylococcus aureus using disk diffusion, well and microdilution techniques. It was observed that EBA inhibited microbial growth in bactericidal concentration ≥156.25μg/mL. The cellular cytotoxicity of EBA, oEd and alpha-humuleno for the lineages HaCat, L929 and RAW 264.7 was determined by employing the colorimetric method 3-(4.5-Dimetiltiazol-2-il) 2.5-diphenyl tetrazolium bromide (MTT) and no cytotoxicity was observed. Inflammatory activity was investigated in vitro by using lipopolysaccharides stimulated macrophages treated with EBA, oEd and alpha-humuleno; all of them exhibited inhibition of nitric oxide production at least 49.87%. The potential of EBA, oEd and alpha-humuleno in promoting cellular migration was analyzed with the lineages HaCat and L929, with emphasis to the essential oil, which promoted the wounds closure 12 hours earlier compared to control. Moreover, for safety purposes, EBA, oEd and formulations were tested in vivo using the Hen's Egg Test – Chorioallantoic Membrane (HET-CAM), concluding those compounds have not jeopardized the chorioallantoic membrane. The HET-CAM assay also verified the EBA, oEd and formulations angiogenic potential, which demonstrated similar action compared to control (Regederm). EBA containing microparticles, that were obtained by spray drying using the chitosan biopolymer, were characterized according to morphology, yield (31.25%), diameter (19.69μm) and Zeta potential (41,1±4,26mV). Those microparticles presented 70.13% encapsulation efficiency and also characteristics suitable for dermatologic use. The emulsion carried EBA maintained the system stability for up to 60 days while under 8°c. The catechin release proceeded progressively for 24 hours. Cutaneous permeation test demonstrated that the emulsion promoted the catechin penetration (13,56μg/cm2 ) into the viable epidermis layer and it is possible to say that the emulsion increases the catechin penetration. Stability studies have shown that the emulsion refrigeration increases the product durability, because they indicated the preservation of catechin content. Therefore, emulsion with EBA containing microparticles may be an alternative option to assist the antimicrobial treatment; while the oil demonstrated better results as anti-inflammatory, reepithelialization promoter and pro-angiogenic, being a potential compound for wound healing, while not promoting irritating effects. The evaluated formulations focus on comfortable patients recovery, leading to better treatment adherence. Furthermore, those formulations can promote the tissue epithelialization throughout the antimicrobial, inflammatory and healing action. It is an innovative and genuinely Brazilian proposal.
metadata.dc.description.unidade:	Faculdade de Ciências da Saúde (FS)
Description:	Tese (Doutorado)—Universidade de Brasília, Faculdade de Ciências da Saúde, 2018.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Ciências da Saúde
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Collection(s) :	Teses, dissertações e produtos pós-doutorado

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