Título :	Proteoma e transcriptoma comparativo das glândulas salivares de barbeiros das espécies de Rhodnius brethesi, Rhodnius robustus e Panstrongylus megistus
Autor :	Maranhão, Ana Carolina Bussacos
Orientador(es)::	Teixeira, Antônio Raimundo Lima Cruz
Assunto::	Chagas, Doença de Tripanossoma cruzi Entomologia
Fecha de publicación :	26-feb-2009
Data de defesa::	2008
Citación :	MARANHÃO, Ana Carolina Bussacos. Proteoma e transcriptoma comparativo das glândulas salivares de barbeiros das espécies de Rhodnius brethesi, Rhodnius robustus e Panstrongylus megistus. 2008. 100 f. Tese (Doutorado em Patologia Molecular)-Universidade de Brasilia, Brasilia, 2008.
Abstract:	Chagas disease caused by the protozoan Trypanosoma cruzi is mostly transmitted by the triatomine bug and causes over 17 million infections in Latin America. More than 140 species of triatomines were described. The Rhodnius gender comprises almost half of the triatomines found in the Amazon Basin, as Rhodnius brethesi and Rhodnius robustus, normally these insects aren’t in contact with humans, but deforestation and migration are approaching the infected bugs to human habitats and working areas. Differently, Panstrongylus megistus is widely distributed from Mexico to Argentina and are known to colonize different wild life dwellings, such as ground burrows, birds nest, crevices on tree barks and human domiciles. In this study we described the transcriptome of the salivary glands of R. brethesi and R. robustus, respectively comprising 56 and 122 clusters. Over 30% of these clusters had never been described before. We also sequenced for the first time 45 transcripts from the P. megistus salivary gland. Furthermore, we performed a proteomic analysis using LC-MS/MS technology and found 123, 111, 159 proteins in R. brethesi, R. robustus and P. megistus proteome, respectively. Besides the highly redundant housekeeping proteins we also could found lipocalins (biogenic amino binding proteins and salivary platelet aggregation inhibitor), inositol polyphosphate 5-phosphatase, and kazal domain proteins, essential proteins used for the successful blood-feeding habits, and glutathione S transferase, and antigen-5 protein, respectively, involved with resistance to insecticide and defense. Rhodnius findings showed their genetic distance with Triatoma, in the opposite, data confirmed the closeness between Triatoma and P. megistus.
metadata.dc.description.unidade:	Faculdade de Medicina (FM)
Descripción :	Tese (doutorado)—Universidade de Brasília, Faculdade de Medicina, 2008.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Patologia Molecular
Aparece en las colecciones:	Teses, dissertações e produtos pós-doutorado

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