Título :	Dynamic ex vivo porcine eye model to measure ophthalmic drug penetration under simulated lacrimal flow
Autor :	Barbalho, Geisa Nascimento Falcão, Manuel Alves Lopes, Jefferson Márcio Sanches Lopes, Júlia M. Contarato, Jonad Logan Adriano Gelfuso, Guilherme Martins Cunha Filho, Marcílio Sérgio Soares da Gratieri, Taís
metadata.dc.identifier.orcid:	https://orcid.org/0000-0002-3092-0137 https://orcid.org/0000-0002-1924-7885 https://orcid.org/0000-0002-9167-6852 https://orcid.org/0000-0001-6481-2364
metadata.dc.contributor.affiliation:	University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC) Federal University of Pará, Physics Department University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC) University of Brasilia, Laboratory of Food, Drugs, and Cosmetics (LTMAC)
Assunto::	Medicamentos - administração Oftalmologia Modelo animal
Fecha de publicación :	15-sep-2023
Editorial :	MDPI
Citación :	BARBALHO, Geisa N. et al. Dynamic ex vivo porcine eye model to measure ophthalmic drug penetration under simulated lacrimal flow. Pharmaceutics, [S.l.], v. 15, n. 9, 15 set. 2023. DOI: https://doi.org/10.3390/pharmaceutics15092325. Disponível em: https://www.mdpi.com/1999-4923/15/9/2325. Acesso em: 23 março 2024.
Abstract:	Animal models are still used in the research and development of ophthalmic drug products, mainly due to the difficulty in simulating natural physiological conditions with in vitro models, as there is a lack of dynamic protection mechanisms. Therefore, developing alternative ophthalmic models that evaluate drug penetration in the cornea while applying dynamic protection barriers is a contemporary challenge. This study aimed to develop a dynamic ex vivo model using porcine eyes with a simulated lacrimal flow to evaluate the performance of pharmaceutical drug products. A glass donor cell to support a simulated tear flow was designed, optimized, and custom-made. The system was challenged with different formulations (with fluconazole) including excipients with different viscosities (poloxamer 407) and mucoadhesive properties (chitosan). The results were compared to those obtained from a conventional excised cornea model mounted in Franz-type diffusion cells. The dynamic model could differentiate formulations, while the static model did not, overestimating ex vivo drug penetrated amounts. Hence, the dynamic model with simulated tear flow showed to be a simple and promising new alternative method for the drug penetration of ophthalmic formulations that ultimately can reduce the number of animals used in research.
metadata.dc.description.unidade:	Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
metadata.dc.description.ppg:	Programa de Pós-Graduação em Ciências Farmacêuticas
Licença::	© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/pharmaceutics15092325
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