Title:	A wor1-like transcription factor is essential for virulence of Cryptococcus neoformans
Authors:	Paes, Hugo Costa Derengowski, Lorena da Silveira Peconick, Luisa Defranco Ferreira Albuquerque, Patrícia Pappas Júnior, Georgios Joannis Nicola, André Moraes Silva, Fabiana Brandão Alves Vallim, Marcelo Afonso Alspaugh, J. Andrew Felipe, Maria Sueli Soares Fernandes, Larissa
metadata.dc.contributor.affiliation:	University of Brasília Medical School, Clinical Medicine Division Military College University of Brasília, Ceilândia College University of Brasília, Ceilândia College University of Brasília, Institute of Biological Sciences, Department of Cell Biology University of Brasília Medical School, Pathology Division Paulista University, Department of Biomedicine São Paulo Federal University, Biological Sciences Department, Cellular and Molecular Biology Division Duke University, School of Medicine, Department of Medicine Catholic University of Brasília, Graduate School in Genomic Sciences
Assunto::	Cryptococcus neoformans Citocinese Virulência
Issue Date:	13-Nov-2018
Publisher:	Frontiers
Citation:	PAES, Hugo Costa et al. A wor1-like transcription factor is essential for virulence of Cryptococcus neoformans. Frontiers in Cellular and Infection Microbiology, [S.l.], v. 8, art. 369, 2018. DOI: https://doi.org/10.3389/fcimb.2018.00369. Disponível em: https://www.frontiersin.org/articles/10.3389/fcimb.2018.00369/full. Acesso em: 04 out. 2023.
Abstract:	Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to species, including regulating morphological transition and virulence, mating and secondary metabolism. Many of these functions are important for fungal pathogenesis. We therefore hypothesized that one of the two proteins of this family in Cryptococcus neoformans, a major pathogen of humans, would also control virulence-associated cellular processes. Elimination of this protein in C. neoformans results in reduced polysaccharide capsule expression and defective cytokinesis and growth at 37◦C. The mutant loses virulence in a mouse model of cryptococcal infection and retains only partial virulence in the Galleria mellonella alternative model at 30◦C. We performed RNA-Seq experiments on the mutant and found abolished transcription of genes that, in combination, are known to account for all the observed phenotypes. The protein has been named Required for cytokinesis and virulence 1 (Rcv1).
metadata.dc.description.unidade:	Faculdade de Medicina (FMD) Faculdade UnB Ceilândia (FCE) Instituto de Ciências Biológicas (IB) Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR) Departamento de Biologia Celular (IB CEL)
Licença::	Copyright © 2018 Paes, Derengowski, Peconick, Albuquerque, Pappas, Nicola, Silva, Vallim, Alspaugh, Felipe and Fernandes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI:	https://doi.org/10.3389/fcimb.2018.00369
Appears in Collections:	Artigos publicados em periódicos e afins

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