Title:	Pouteria macrophylla fruit extract microemulsion for cutaneous depigmentation : evaluation using a 3D pigmented skin model
Authors:	Brathwaite, Ana Clara Nogueira Silva, Thuany Alencar Carvalho, Larissa A. C. Branquinho, Maryana Stephany Ferreira Nunes, Ricardo Ferreira Cunha Filho, Marcílio Sérgio Soares da Gelfuso, Guilherme Martins Maria-Engler, Silvya Stuchi Carvalho, Juliana Lott de Silva, Joyce Kelly do Rosário da Gratieri, Taís
metadata.dc.contributor.email:	mailto:tgratieri@gmail.com
metadata.dc.identifier.orcid:	https://orcid.org/0000-0003-2637-9269 https://orcid.org/0000-0002-9167-6852 https://orcid.org/0000-0002-1924-7885 https://orcid.org/0000-0003-4771-6041 https://orcid.org/0000-0002-1423-0523 https://orcid.org/0000-0002-9351-876X https://orcid.org/0000-0001-6481-2364
Assunto::	Extrato de cutita Ácido gálico Hiperpigmentação
Issue Date:	14-Sep-2022
Publisher:	MDPI
Citation:	BRATHWAITE, Ana Clara N. et al. Pouteria macrophylla fruit extract microemulsion for cutaneous depigmentation: evaluation using a 3D pigmented skin model. Molecules, Basel, v. 27, n. 18, art. 5982, 2022. DOI 10.3390/molecules27185982. Disponível em: https://www.mdpi.com/1420-3049/27/18/5982. Acesso em: 03 nov. 2022.
Abstract:	Here, we verify the depigmenting action of Pouteria macrophylla fruit extract (EXT), incorporate it into a safe topical microemulsion and assess its effectiveness in a 3D pigmented skin model. Melanocytes-B16F10- were used to assess the EXT effects on cell viability, melanin synthesis, and melanin synthesis-related gene transcription factor expression, which demonstrated a 32% and 50% reduction of intra and extracellular melanin content, respectively. The developed microemulsion was composed of Cremophor EL®/Span 80 4:1 (w/w), ethyl oleate, and pH 4.5 HEPES buffer and had an average droplet size of 40 nm (PdI 0.40 ± 0.07). Skin irritation test with reconstituted epidermis (Skin Ethic RHETM) showed that the formulation is non-irritating. Tyrosinase inhibition was maintained after skin permeation in vitro, in which microemulsion showed twice the inhibition of the conventional emulsion (20.7 ± 2.2% and 10.7 ± 2.4%, respectively). The depigmenting effect of the microemulsion was finally confirmed in a 3D culture model of pigmented skin, in which histological analysis showed a more pronounced effect than a commercial depigmenting formulation. In conclusion, the developed microemulsion is a promising safe formulation for the administration of cutite fruit extract, which showed remarkable depigmenting potential compared to a commercial formulation.
metadata.dc.description.unidade:	Faculdade de Ciências da Saúde (FS)
Licença::	Molecules - No special permission is required to reuse all or part of article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. Reuse of an article does not imply endorsement by the authors or MDPI. Fonte: https://www.mdpi.com/openaccess. Acesso em: 03 nov. 2022.
DOI:	https://doi.org/10.3390/molecules27185982
Appears in Collections:	Artigos publicados em periódicos e afins

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