Titre:	Faster cryptococcus melanization increases virulence in experimental and human cryptococcosis
Auteur(s):	Sousa, Herdson Renney de Oliveira Júnior, Getúlio Pereira de Frazão, Stefânia de Oliveira Gorgonha, Kaio César de Melo Rosa, Camila Pereira Melgaço Garcez, Emãnuella Lucas Júnior, Joaquim Correia, Amabel Fernandes Freitas, Waleriano Ferreira de Borges, Higor Matos Alves, Lucas Gomes de Brito Paes, Hugo Costa Trilles, Luciana Lazera, Márcia dos Santos Teixeira, Marcus de Melo Pinto Júnior, Vitor Laerte Felipe, Maria Sueli Soares Casadevall, Arturo Pereira, Ildinete Silva Albuquerque, Patrícia Nicola, André Moraes
metadata.dc.identifier.orcid:	https://orcid.org/ 0000-0001-6940-401X https://orcid.org/ 0000-0002-1598-4191 https://orcid.org/ 0000-0002-8369-8680 https://orcid.org/ 0000-0002-8369-8680 https://orcid.org/ 0000-0001-8932-3954 https://orcid.org/ 0000-0003-1763-3464 https://orcid.org/ 0000-0003-0556-5310 https://orcid.org/ 0000-0002-9402-9167 https://orcid.org/ 0000-0001-8160-7784
Assunto::	Cryptococcus neoformans Criptococose Virulência (Microbiologia) Melanina Fagocitose
Date de publication:	12-avr-2022
Editeur:	MDPI
Référence bibliographique:	SOUSA, Herdson Renney de et al. Faster cryptococcus melanization increases virulence in experimental and human cryptococcosis. Journal of Fungi, v. 8, n. 4, 393, 2022. DOI: https://doi.org/10.3390/jof8040393. Disponível em: https://www.mdpi.com/2309-608X/8/4/393. Acesso em: 08 jun. 2022.
Abstract:	Cryptococcus spp. are human pathogens that cause 181,000 deaths per year. In this work, we systematically investigated the virulence attributes of Cryptococcus spp. clinical isolates and correlated them with patient data to better understand cryptococcosis. We collected 66 C. neoformans and 19 C. gattii clinical isolates and analyzed multiple virulence phenotypes and host–pathogen interaction outcomes. C. neoformans isolates tended to melanize faster and more intensely and produce thinner capsules in comparison with C. gattii. We also observed correlations that match previous studies, such as that between secreted laccase and disease outcome in patients. We measured Cryptococcus colony melanization kinetics, which followed a sigmoidal curve for most isolates, and showed that faster melanization correlated positively with LC3-associated phagocytosis evasion, virulence in Galleria mellonella and worse prognosis in humans. These results suggest that the speed of melanization, more than the total amount of melanin Cryptococcus spp. produces, is crucial for virulence.
metadata.dc.description.unidade:	Faculdade de Medicina (FMD)
Licença::	Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)
DOI:	https://doi.org/10.3390/jof8040393
Collection(s) :	Artigos publicados em periódicos e afins UnB - Professores Eméritos

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