Title:	Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential
Authors:	Moura, Ágata Nogueira D’Áurea Oliveira, Diane Sthefany Lima de Paredes, Verenice Rocha, Letícia Barboza Oliveira, Fabiana Freire Mendes de Lessa, Gustavo Meirelles Riasco Palacios, Juan Fernando Casadevall, Arturo Albuquerque, Patrícia Felipe, Maria Sueli Soares Piazza, Roxane Maria Fontes Nicola, André Moraes
metadata.dc.identifier.orcid:	https://orcid.org/ 0000-0003-0480-5028 https://orcid.org/ 0000-0002-2655-4672 https://orcid.org/ 0000-0001-7832-058X https://orcid.org/ 0000-0002-9170-1034 https://orcid.org/ 0000-0002-9402-9167 https://orcid.org/ 0000-0001-8160-7784 https://orcid.org/ 0000-0001-8656-5835
Assunto::	Anticorpos Micoses Infecção - fungos Tratamento
Issue Date:	28-Sep-2020
Publisher:	MDPI
Citation:	MOURA, Ágata Nogueira D’Áurea et al. Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential. Journal Fungi, v. 6, n. 4, 193, 2020. DOI: https://doi.org/10.3390/jof6040193. Disponível em: https://www.mdpi.com/2309-608X/6/4/193. Acesso em: 14 abr. 2022.
Abstract:	Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in Latin America. It affects mainly male rural workers in impoverished regions, and the therapy can last up to two years or use drugs that are very toxic. Given the need for novel safe and effective approaches to treat PCM, we have been developing monoclonal antibodies (mAbs) that could be used not only to block specific fungal targets, but also modulate the host’s antifungal immunity. In this work we show the generation of and promising results with an mAb against Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very immunogenic and both the polyclonal serum and mAb were used in immunofluorescence experiments, which showed binding of antibodies to the yeast cell surface. The mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations with J774.16 macrophage-like cells. Our results suggest that this mAb could serve as the basis for new immunotherapy regimens for PCM.
metadata.dc.description.unidade:	Faculdade de Medicina (FMD)
DOI:	https://doi.org/10.3390/jof6040193
Appears in Collections:	Artigos publicados em periódicos e afins UnB - Professores Eméritos

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