Title:	Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism
Authors:	Mayer, Andréia Biolchi Oliveira, Danilo Gustavo Rodrigues de Amaral, Henrique de Oliveira Campos, Gabriel Avohay Alves Gonçalves, Jacqueline Coimbra Souza, Adolfo Carlos Barros de Lima, Marcos Robalinho Silva, Luciano Paulino Mortari, Márcia Renata
metadata.dc.identifier.orcid:	https://orcid.org/0000-0002-5901-0911 https://orcid.org/0000-0001-9075-7725
Assunto::	Vespa - veneno Capacidade motora Parkinson, Doença de
Issue Date:	27-Aug-2020
Publisher:	MDPI
Citation:	BIOLCH, Andréia Mayer et al. Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism. Toxins, v. 12, n. 9, 550, 2020. DOI: https://doi.org/10.3390/toxins12090550. Disponível em: https://www.mdpi.com/2072-6651/12/9/550. Acesso em: 11 nov. 2020.
Abstract:	Parkinson’s disease (PD) is a progressive neurodegenerative condition that affects the Central Nervous System (CNS). Insect venoms show high molecular variability and selectivity in the CNS of mammals and present potential for the development of new drugs for the treatment of PD. In this study, we isolated and identified a component of the venom of the social wasp Parachartergus fraternus and evaluated its neuroprotective activity in the murine model of PD. For this purpose, the venom was filtered and separated through HPLC; fractions were analyzed through mass spectrometry and the active fraction was identified as a novel peptide, called Fraternine. We performed two behavioral tests to evaluate motor discoordination, as well as an apomorphine-induced rotation test. We also conducted an immunohistochemical assay to assess protection in TH+ neurons in the Substantia Nigra (SN) region. Group treated with 10 µg/animal of Fraternine remained longer in the rotarod compared to the lesioned group. In the apomorphine test, Fraternine decreased the number of rotations between treatments. This dose also inhibited dopaminergic neuronal loss, as indicated by immunohistochemical analysis. This study identified a novel peptide able to prevent the death of dopaminergic neurons of the SN and recover motor deficit in a 6-OHDA-induced murine model of PD.
metadata.dc.description.unidade:	Faculdade de Medicina (FMD)
Licença::	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI:	https://doi.org/10.3390/toxins12090550
Appears in Collections:	Artigos publicados em periódicos e afins

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