Título:	Production of Brazilian human norovirus VLPs and comparison of purification methods
Autor(es):	Lamounier, Thaís Alves da Costa Oliveira, Layssa Miranda de Camargo, Brenda Rabello de Rodrigues, Kelly Barreto Noronha, Eliane Ferreira Ribeiro, Bergmann Morais Nagata, Tatsuya
Assunto:	Vírus Cromatografia de troca iônica
Data de publicação:	Out-2015
Editora:	Sociedade Brasileira de Microbiologia
Referência:	LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925.
Abstract:	Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Licença:	Brazilian Journal of Microbiology - All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso. Acesso em: 2 abr. 2018.
DOI:	http://dx.doi.org/10.1590/S1517-838246420140925
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