Título:	Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
Autor(es):	Romero, Gustavo Adolfo Sierra Sampaio, Raimunda Nonata Ribeiro Macêdo, Vanize de Oliveira Marsden, Philip Davis
Assunto:	Leishmania braziliensis Contaminação fúngica Leishmaniose cutânea
Data de publicação:	Jul-1999
Editora:	Instituto Oswaldo Cruz, Ministério da Saúde
Referência:	ROMERO, Gustavo Adolfo Sierra et al. Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis transmission. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 4, p. 505-508, jul. 1999. DOI: https://doi.org/10.1590/S0074-02761999000400014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014&lng=en&nrm=iso. Acesso em: 12 jan. 2021.
Abstract:	Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.
Unidade Acadêmica:	Faculdade de Medicina (FMD)
Licença:	Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014&lng=en&tlng=en. Acesso em: 12 jan. 2021.
DOI:	https://dx.doi.org/10.1590/S0074-02761999000400014
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