Titre:	Characterization of a protease produced by a Trichoderma harzianum isolate which controls cocoa plant witches' broom disease
Auteur(s):	De Marco, Janice Lisboa Felix, Carlos Roberto
Assunto::	Enzimas de fungos Enzimas proteolíticas Cacaueiro - doenças e pragas Fungos como agentes no controle biológico de pragas
Date de publication:	22-jan-2002
Editeur:	BioMed Central
Référence bibliographique:	MARCO, Janice Lisboa De; FELIX, Carlos Roberto. Characterization of a protease produced by a Trichoderma harzianum isolate which controls cocoa plant witches' broom disease. BMC Biochemistry, v. 3, Article 3, 22 jan. 2002. Disponível em: <https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-3-3>. Acesso em: 22 jun. 2017. doi: https://doi.org/10.1186/1471-2091-3-3.
Abstract:	Background: Several Trichoderma strains have been reported to be effective in controlling plant diseases, and the action of fungal hydrolytic enzymes has been considered as the main mechanism involved in the antagonistic process. However, although Trichoderma strains were found to impair development of Crinipellis perniciosa, the causal agent of cocoa plant witches' broom disease, no fungal strain is available for effective control of this disease. We have then undertaken a program of construction of hydrolytic enzyme-overproducing Trichoderma strains aiming improvement of the fungal antagonistic capacity. The protease of an indian Trichoderma isolate showing antagonistic activity against C. perniciosa was purified to homogeneity and characterized for its kinetic properties and action on the phytopathogen cell wall. Results: A protease produced by the Trichoderma harzianum isolate 1051 was purified to homogeneity by precipitation with ammonium sulfate followed by hydrophobic chromatography. The molecular mass of this protease as determined by SDS-polyacrylamide gel electrophoresis was about 18.8 kDa. Its N-terminal amino acid sequence shares no homology with any other protease. The purified enzyme substantially affected the cell wall of the phytopathogen C. perniciosa. Westernblotting analysis showed that the enzyme was present in the culture supernatant 24 h after the Trichoderma started to grow in casein-containing liquid medium. Conclusions: The capacity of the Trichoderma harzianum protease to hydrolyze the cell wall of C. perniciosa indicates that this enzyme may be actually involved in the antagonistic process between the two fungi. This fact strongly suggest that hydrolytic enzyme over-producing transgenic fungi may show superior biocontrol capacity.
Licença::	© 2002 De Marco and Felix; licensee BioMed Central Ltd. Verbatim copying and redistribution of this article are permitted in any medium for any purpose, provided this notice is preserved along with the article's original URL.
DOI:	https://dx.doi.org/10.1186/1471-2091-3-3
Collection(s) :	Artigos publicados em periódicos e afins

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