Skip navigation
Universidade de Brasília
Use este identificador para citar ou linkar para este item: http://repositorio.unb.br/handle/10482/7892
Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
ARTIGO_DetectionThreeAllexivirus.PDF1,23 MBAdobe PDFVisualizar/Abrir
Título: Detection of three Allexivirus species infecting garlic in Brazil
Outros títulos: Detecção de três espécies de Allexivirus que infectam o alho no Brasil
Autor(es): Melo Filho, Péricles de Albuquerque
Nagata, Tatsuya
Dusi, André Nepomuceno
Buso, José Amauri
Torres, Antonio Carlos
Eiras, Marcelo
Resende, Renato de Oliveira
Assunto: Alho
Hortaliças - doenças e pragas
Data de publicação: Ago-2004
Referência: MELO FILHO, Péricles de Albuquerque et al. Detection of three Allexivirus species infecting garlic in Brazil. Pesquisa Agropecuária Brasileira, Brasília, v. 39, n. 8, p. 735-740, ago. 2004. Disponível em: <http://www.scielo.br/pdf/pab/v39n8/21733.pdf>. Acesso em: 26 abr. 2011. doi: http://dx.doi.org/10.1590/S0100-204X2004000800002.
Abstract: Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil.
DOI: http://dx.doi.org/10.1590/S0100-204X2004000800002.
Aparece nas coleções:Artigos publicados em periódicos e afins

Mostrar registro completo do item Visualizar estatísticas



Este item está licenciada sob uma Licença Creative Commons Creative Commons