Colorimetric LAMP assay for detection of Xanthomonas phaseoli pv. manihotis in Cassava through genomics : a new approach to an old problem

Carvalho, Ian Carlos Bispo de; Carvalho, Alice Maria Silva; Wendland, Adriane; Rossato, Maurício

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Campo DC	Valor	Idioma
dc.contributor.author	Carvalho, Ian Carlos Bispo de	-
dc.contributor.author	Carvalho, Alice Maria Silva	-
dc.contributor.author	Wendland, Adriane	-
dc.contributor.author	Rossato, Maurício	-
dc.date.accessioned	2026-11-11T15:48:16Z	-
dc.date.available	2026-11-11T15:48:16Z	-
dc.date.issued	2024-09	-
dc.identifier.citation	CARVALHO, Ian C. Bispo et al. Colorimetric LAMP assay for detection of Xanthomonas phaseoli pv. manihotis in Cassava through genomics: a new approach to an old problem. Plant Disease, v. 108, n. 10, 2024. DOI: https://doi.org/10.1094/PDIS-08-23-1507-R. Disponível em: https://apsjournals.apsnet.org/doi/full/10.1094/PDIS-08-23-1507-RE. Acesso em: 11 nov. 2026.	pt_BR
dc.identifier.uri	http://repositorio.unb.br/handle/10482/57399	-
dc.language.iso	eng	pt_BR
dc.publisher	The American Phytopathological Society (APS)	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.title	Colorimetric LAMP assay for detection of Xanthomonas phaseoli pv. manihotis in Cassava through genomics : a new approach to an old problem	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Mandioca	pt_BR
dc.subject.keyword	Diagnóstico molecular	pt_BR
dc.subject.keyword	Bactérias patogênicas	pt_BR
dc.subject.keyword	Amplificação isotérmica mediada por alças (LAMP)	pt_BR
dc.identifier.doi	https://doi.org/10.1094/PDIS-08-23-1507-R	pt_BR
dc.relation.publisherversion	https://apsjournals.apsnet.org/doi/full/10.1094/PDIS-08-23-1507-RE	pt_BR
dc.description.abstract1	Bacterial blight caused by Xanthomonas phaseoli pv. manihotis (Xpm) is considered the main bacterial disease that affects cassava, causing significant losses when not properly managed. In the present study, a fast, sensitive, and easy-to-apply method to detect Xpm via colorimetric loop-mediated isothermal amplification (LAMP) was developed. To ensure the use of a unique-to-the-target pathovar core region for primer design, 74 complete genomic sequences of Xpm together with different bacterial species and pathovars were used for comparative genomics. A total of 42 unique genes were used to design 27 LAMP primer sets, from which nine primers were synthesized, and only one (Xpm_Lp1 primer set) showed sufficient efficiency in preliminary tests. The sensitivity, assessed by a serial dilution of the type strain (IBSBF 278) DNA, yielded high sensitivity, detecting up to 100 fg. The LAMP primers showed high specificity, did not cross-react with other bacterial species or other pathovars tested, and amplified only the Xpm isolates. Tests confirmed the high efficiency of the protocol using infected or inoculated macerated cassava leaves without the need for additional sample treatment. The LAMP test developed in this study was able to detect Xpm in a fast, simple, and sensitive way, and it can be used to monitor the disease under laboratory and field conditions.	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0002-8817-7374	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0002-0064-8849	pt_BR
dc.contributor.affiliation	Universidade de Brasília	pt_BR
dc.contributor.affiliation	Universidade de Brasília	pt_BR
dc.contributor.affiliation	Embrapa Arroz e Feijão, Goiânia, Goás, Brazil	pt_BR
dc.contributor.affiliation	Universidade de Brasília	pt_BR
dc.description.unidade	Instituto de Ciências Biológicas (IB)	pt_BR
dc.description.unidade	Departamento de Fitopatologia (IB FIT)	pt_BR
dc.description.ppg	Programa de Pós-Graduação em Fitopatologia	pt_BR
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