Título:	Estimulação cerebral profunda precoce no modelo da doença de Parkinson induzida pela infusão intraestriatal de 6-hidroxidopamina em camundongos
Autor(es):	Ruiz, Miguel Cesar Merino
Orientador(es):	Mortari, Márcia Renata
Coorientador(es):	Goulart, Jair Trapé
Assunto:	Neuroproteção Estimulação cerebral Parkinson, Doença de 6-OHDA Roedores
Data de publicação:	6-Jun-2026
Data de defesa:	19-Jul-2024
Referência:	RUIZ, Miguel Cesar Merino. Estimulação cerebral profunda precoce no modelo da doença de Parkinson induzida pela infusão intraestriatal de 6-hidroxidopamina em camundongos. 2024. 156 f., il. Tese (Doutorado em Ciências da Saúde) — Universidade de Brasília, Brasília, 2024.
Abstract:	Deep brain stimulation (DBS) of brain nuclei is an effective symptomatic therapeutic strategy for the treatment of Parkinson's disease (PD). Unlike in humans, there is evidence of DBS mediated neuroprotection in rodents in which parkinsonism was experimentally induced in protocols conducted outside the acute phase of experimental disease induction. This study aims to investigate motor responses and neuroprotection resulting from DBS in the acute phase of parkinsonism induction through intrastriatal injection of the neurotoxin 6-hydroxydopamine (6- OHDA) in mice. Three experimental groups were defined: the 6-OHDA+DBS group, which received a left nigrostriatal lesion and the implantation of an activated ipsilateral intracerebral DBS electrode to neuromodulate the Subthalamic Nucleus (STN) (n=6); the 6-OHDA group, which underwent the same procedure, but the electrode remained inactive (n=8); and the Naive group, with no interventions (n=5). The experimental protocol occurred on day zero (D0). During the initial four days following the procedures (D1 to D4), behavioral assessments were conducted across the three groups, alongside measurement of the electrical resistance of the brain-electrode system in the 6-OHDA+DBS group. The animals' body mass was measured from D0 to D4 and on D7, immediately before euthanasia. Additionally, the groups underwent an immunohistochemical study using Tyrosine Hydroxylase (TH) staining on sections of the Substantia Nigra (SN) and striatum to quantify differences in the number of nigral dopaminergic neurons and the density of dopaminergic terminals in the striatum between the lesioned and intact sides. Despite the animals' poor clinical condition during the acute phase of parkinsonism induction, those that underwent four days of DBS showed an increase in body mass, favorable motor effects, a reduction in the loss of striatal dopaminergic terminals, and both global and compartmentalized neuroprotective effects in the SN compared to those that did not. The deep brain stimulation device developed for this study was cost-effective, with a suitable design for cranial anchoring and dimensions appropriate for the mouse brain. In the current pursuit of diagnosing and treating PD in pre-motor stages, these findings contribute to enriching the discussion on neuroprotective therapeutic strategies, suggesting invasive neuromodulation as a potentially useful therapeutic tool within an appropriate context.
Unidade Acadêmica:	Faculdade de Ciências da Saúde (FS)
Informações adicionais:	Tese (doutorado) — Universidade de Brasília, Faculdade de Ciências da Saúde, Programa de Pós-Graduação em Ciências da Saúde, 2024.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências da Saúde
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) e Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF)
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.