Título :	Evaluation of different cryoprotectant combinations in vitrification and slow freezing for ovarian tissue preservation in domestic cats
Autor :	Ribeiro, Rayane Brandão Rodrigues, Aline de Queiroz Aguiar, Beatriz Alves de Silva, Jessyca Karoline Oliveira Costa, Marcus Vinicius Rocha de a Carvalho da Bezerra, Julliene Larissa dos Santos Ferreira, Yasmin Barboza Silva, Ingrid Gracielle Martins da Piau, Tathyana Benetis Lucci, Carolina Madeira Báo, Sônia Nair Goulart, Jair Trapé Bellozi, Paula Maria Quaglio Paulini, Fernanda
metadata.dc.contributor.affiliation:	University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Cellular Biology University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Cellular Biology University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Faculty of Health Sciences, Molecular Pharmacology Laboratory University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences
Assunto::	Reprodução animal Criopreservação Felinos Folículos ovarianos
Fecha de publicación :	abr-2025
Editorial :	Wiley
Citación :	RIBEIRO, Rayane Brandão et al. Evaluation of different cryoprotectant combinations in vitrification and slow freezing for ovarian tissue preservation in domestic cats. Cats. Reprod Dom Anim, [S. l.], v. 60, 2025. DOI: https://doi.org/10.1111/rda.70064. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/rda.70064. Acesso em: 11 mar. 2026.
Abstract:	Over the past decade, increased hunting and habitat disturbance have significantly impacted the endangered population within the Felidae family. Recognising this, it becomes imperative to implement strategies aimed at mitigating this concerning conservation scenario. For this, female fertility preservation is crucial in this context, and studies concerning this field are still scarce. In the realm of cryopreservation, prevalent methods involve slow freezing (SF) and vitrification (V). This study aimed to evaluate various cryoprotective combinations for V or SF processes applied to domestic cat ovarian tissue. Twenty ovaries from 10 healthy cats were dissected, and cortical regions were sectioned into eight fragments measuring 3 mm3 each. These fragments were randomly allocated to three different treatment groups for V (V1, V2 and V3) or SF (SF1, SF2 and SF3). Each group employed solutions with varying concentrations of DMSO, EG and either trehalose or sucrose. The assessment included histological evaluation, follicle counting, immunohistochemical analysis of proliferative activity, and ultrastructural examination. The results demonstrated that the V1 protocol—composed of an equilibration solution with 10% DMSO, 10% EG and 0.1 M trehalose, followed by a V solution with 20% DMSO, 20% EG and 0.1 M trehalose—proved most effective. This combination best preserved follicular morphology, reduced degeneration, supported follicle proliferation and maintained favourable ultrastructural integrity compared to other treatments. These findings provide a valuable foundation for improving fertility preservation in domestic cats, with potential applications for endangered felid conservation programs.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Ciências Fisiológicas (IB CFS) Departamento de Biologia Celular (IB CEL) Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
metadata.dc.description.ppg:	Programa de Pós-Graduação em Biologia Animal
Licença::	This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
DOI:	https://doi.org/10.1111/rda.70064
metadata.dc.relation.publisherversion:	https://onlinelibrary.wiley.com/doi/10.1111/rda.70064
Aparece en las colecciones:	Artigos publicados em periódicos e afins

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.