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dc.contributor.authorRibeiro, Rayane Brandão-
dc.contributor.authorRodrigues, Aline de Queiroz-
dc.contributor.authorAguiar, Beatriz Alves de-
dc.contributor.authorSilva, Jessyca Karoline Oliveira-
dc.contributor.authorCosta, Marcus Vinicius Rocha de a Carvalho da-
dc.contributor.authorBezerra, Julliene Larissa dos Santos-
dc.contributor.authorFerreira, Yasmin Barboza-
dc.contributor.authorSilva, Ingrid Gracielle Martins da-
dc.contributor.authorPiau, Tathyana Benetis-
dc.contributor.authorLucci, Carolina Madeira-
dc.contributor.authorBáo, Sônia Nair-
dc.contributor.authorGoulart, Jair Trapé-
dc.contributor.authorBellozi, Paula Maria Quaglio-
dc.contributor.authorPaulini, Fernanda-
dc.date.accessioned2026-03-11T16:32:48Z-
dc.date.available2026-03-11T16:32:48Z-
dc.date.issued2025-04-
dc.identifier.citationRIBEIRO, Rayane Brandão et al. Evaluation of different cryoprotectant combinations in vitrification and slow freezing for ovarian tissue preservation in domestic cats. Cats. Reprod Dom Anim, [S. l.], v. 60, 2025. DOI: https://doi.org/10.1111/rda.70064. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/rda.70064. Acesso em: 11 mar. 2026.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/54234-
dc.language.isoengpt_BR
dc.publisherWileypt_BR
dc.rightsAcesso Abertopt_BR
dc.titleEvaluation of different cryoprotectant combinations in vitrification and slow freezing for ovarian tissue preservation in domestic catspt_BR
dc.typeArtigopt_BR
dc.subject.keywordReprodução animalpt_BR
dc.subject.keywordCriopreservaçãopt_BR
dc.subject.keywordFelinospt_BR
dc.subject.keywordFolículos ovarianospt_BR
dc.rights.licenseThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.pt_BR
dc.identifier.doihttps://doi.org/10.1111/rda.70064pt_BR
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/rda.70064pt_BR
dc.description.abstract1Over the past decade, increased hunting and habitat disturbance have significantly impacted the endangered population within the Felidae family. Recognising this, it becomes imperative to implement strategies aimed at mitigating this concerning conservation scenario. For this, female fertility preservation is crucial in this context, and studies concerning this field are still scarce. In the realm of cryopreservation, prevalent methods involve slow freezing (SF) and vitrification (V). This study aimed to evaluate various cryoprotective combinations for V or SF processes applied to domestic cat ovarian tissue. Twenty ovaries from 10 healthy cats were dissected, and cortical regions were sectioned into eight fragments measuring 3 mm3 each. These fragments were randomly allocated to three different treatment groups for V (V1, V2 and V3) or SF (SF1, SF2 and SF3). Each group employed solutions with varying concentrations of DMSO, EG and either trehalose or sucrose. The assessment included histological evaluation, follicle counting, immunohistochemical analysis of proliferative activity, and ultrastructural examination. The results demonstrated that the V1 protocol—composed of an equilibration solution with 10% DMSO, 10% EG and 0.1 M trehalose, followed by a V solution with 20% DMSO, 20% EG and 0.1 M trehalose—proved most effective. This combination best preserved follicular morphology, reduced degeneration, supported follicle proliferation and maintained favourable ultrastructural integrity compared to other treatments. These findings provide a valuable foundation for improving fertility preservation in domestic cats, with potential applications for endangered felid conservation programs.pt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Cellular Biologypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Cellular Biologypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Faculty of Health Sciences, Molecular Pharmacology Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Ciências Fisiológicas (IB CFS)pt_BR
dc.description.unidadeDepartamento de Biologia Celular (IB CEL)pt_BR
dc.description.unidadeFaculdade de Ciências da Saúde (FS)pt_BR
dc.description.unidadeDepartamento de Farmácia (FS FAR)pt_BR
dc.description.ppgPrograma de Pós-Graduação em Biologia Animalpt_BR
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