Título:	Influência de parâmetros biológicos na suscetibilidade de Phlebotominae (Diptera: Psychodidae) a inseticidas
Outros títulos:	Influence of biological parameters on the susceptibility of Phlebotominae (Diptera: Psychodidae) to insecticides
Autor(es):	Rocha, Douglas de Almeida
Orientador(es):	Obara, Marcos Takashi
Coorientador(es):	Pessoa, Grasielle Caldas D’Ávila
Assunto:	Flebotomíneo Leishmaniose Controle de vetores Bioensaio Inseticidas - resistência
Data de publicação:	23-Abr-2021
Data de defesa:	25-Set-2020
Referência:	ROCHA, Douglas de Almeida. Influência de parâmetros biológicos na suscetibilidade de Phlebotominae (Diptera: Psychodidae) a inseticidas. 2020. 257 f., il. Tese (Doutorado em Medicina Tropical)—Universidade de Brasília, Brasília, 2020.
Abstract:	Introduction: The classification of insecticide-resistant sandfly populations is based on concepts and methodologies used to characterize the susceptibility profile in mosquitoes. However, sandflies are insects with distinct biology which could lead to erroneous and subjective interpretations in classifying a population to insecticides. Objective: To evaluate the impact of biological variables on the susceptibility of the sandfly Lutzomyia longipalpis to the alpha- pipermethrin insecticide, as well as to identify and describe the reports of sandfly resistance to insecticides in the world and suggest a reference population for future biological tests. Methodology: Lu. longipalpis species were evaluated in relation to sex, age, nutritional status, mortality criteria and sub-lethal effect in the methodologies of CDC bottles and modified WHO cones for the insecticides alpha-cypermethrin and bendiocarb and the diluents alcohol and acetone. In addition, 61 studies, obtained systematically from four scientific databases, were included for evaluation. In addition, three sandfly populations were analyzed for the designation of a reference population. Results: The Kaplan-Meier survival curves showed a statistical difference in the diet of sandflies, in the bottle methodology, where females fed with blood were more susceptible when compared to females fed only with sugary solution using the bottle method (p = 0.001) and age, in the modified cone methodology, in which five-day-old sandflies were more susceptible than three-day-old sandflies (p = 0.001). Gender, alcohol and acetone did not influence the results of biological tests. Records of phlebotomine resistance are described for all classes of insecticides, mainly for the species Phlebotomus papatasi and Ph. argentipes, from the Old World. However, only two studies carried out comparisons between field phlebotomines and xx phlebotomines kept in the laboratory (control) and the phlebotomine doses and lethal times are very varied, revealing that there is no specific protocol for assessing the phlebotomine susceptibility to insecticides. Conclusions: It is concluded that the susceptibility of sandflies to insecticide is influenced by age and nutritional status. However, the sex of insects does not seem to impact the analysis. Currently, there are 47 populations of Phlebotominae with confirmed resistance to one or more insecticides and another 28 populations considered tolerant. In addition, the population of Lu. longipalpis originating from Jacobina and created at Fiocruz in Rio de Janeiro, here it is suggested as a Brazilian Susceptibility Reference Lineage.
Unidade Acadêmica:	Faculdade de Medicina (FM)
Informações adicionais:	Tese (doutorado)—Universidade de Brasília, Faculdade de Medicina, Programa de Pós-Graduação em Medicina Tropical, 2020.
Programa de pós-graduação:	Programa de Pós-Graduação em Medicina Tropical
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) e Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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