Title:	Terapia fotodinâmica como uso de nanoparticulas de nitreto de carbono e conjugados : um estudo in vitro
Authors:	Brito Neta, Maria de Sousa
metadata.dc.contributor.email:	netamsb@hotmail.com
Orientador(es)::	Chaves, Sacha Braun
Assunto::	Câncer - tratamento Nitreto de carbono Espécies Reativas de Oxigênio (ERO) Terapia fotodinâmica
Issue Date:	20-Sep-2020
Data de defesa::	27-Feb-2018
Citation:	BRITO NETA, Maria de Sousa. Terapia fotodinâmica como uso de nanoparticulas de nitreto de carbono e conjugados: um estudo in vitro. 2018. 117 f., il. Dissertação (Mestrado em Nanociência e Nanobiotecnologia)—Universidade de Brasília, Brasília, 2018.
Abstract:	The evolution of medical science has created a demand for more sensitive and selective tools, and nanomedicine offers possibilities of applications of nanomaterials in detection, diagnosis and eventually therapy of pathologies. For cancer treatment, carbon nanomaterials have been considered as promising because their low toxicity, biocompatibility, high solubility in aqueous solutions and spectroscopic properties. Nanoparticles of Carbon Nitride (NP g-C3N4) have photocatalytic properties such as water molecule breakdown and Reactive Oxygen Species (ROS), which make them interesting for applications in Photodynamic Therapy (PDT). In recent years, PDT has been widely used for cancer treatment because it is less invasive than conventional treatments. In this study, use of metal ion-associated NP g-C3N4 as sensitizing agents for PDT in cell lines was evaluated in vitro. Initially, a cell viability assay was performed using 9 materials: 4 nanoparticles (C3N4) associated with metals (Iron, Silver, Manganese and Copper); 1 of nano pure (C3N4) and 4 of free metals. In MTT assay, the one that presented the best result was used for tests with PDT, with ambient light and radiation and light and radiation, as well as incorporation and irradiation time. For particle characterization, it was performed a RAMAN microscopy. MTT results show that cell viability for the B16F10 lineage was significantly reduced when treated with 0.25mg / ml NP g-C3N4 with Cu2 + under blue light irradiation for 10 minutes. In normal cells, Hacat and NIH3T3, treatment of PDT with NPs and blue light irradiation showed no significant toxicity. These results indicate that Cu2 + -containing g-C3N4 NPs can be studied for future applications as selective PDT sensitizing agents for the treatment of cancer.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Genética e Morfologia (IB GEM)
Description:	Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Pós-Graduação em Nanociência e Nanobiotecnologia, 2018.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Nanociência e Nanobiotecnologia
Licença::	A concessão da licença desta coleção refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições:Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Appears in Collections:	Teses, dissertações e produtos pós-doutorado

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