Title:	Efeitos da criopreservação na viabilidade de fibroblastos de felinos silvestres
Authors:	Arantes, Letícia Gobbi
Orientador(es)::	Báo, Sônia Nair
Assunto::	Felinos silvestres Germoplasma Criopreservação de órgãos, tecidos, etc.
Issue Date:	4-Jan-2019
Data de defesa::	27-Jul-2018
Citation:	ARANTES, Letícia Gobbi. Efeitos da criopreservação na viabilidade de fibroblastos de felinos silvestres. 2018. 59 f., il. Dissertação (Mestrado em Biologia Animal)-Universidade de Brasília, Brasília, 2018.
Abstract:	Cryopreservation of genetic material from wild animals, known as germplasm banks, are a viable form of species conservation and biological diversity safeguarding measure. Development of suitable cryopreservation protocols for each cell type is highly necessary considering genetic information banks. This study aims to determine which protocol is best at maintaining cell viability on fibroblasts of the following species; Jaguar (Panthera onca), Northern Tiger Cat (Leopardus tigrinus) and Pampas Cat (Leopardus colocolo) and characterize these cells morphologically. Morphology was examined under Light microscopy, Transmission Electron Microscopy and Scanning Electron Microscopy. Fibroblasts were isolated from skin biopsy and were already cryopreserved on liquid nitrogen (- 196ºC). After thawing, the growth curve was determined for growth potential in eigth days. None of the species reached confluence before the eighth day. Cultured cells presented exponential growth until the first 50 hours. After this period, reduction of growth speed was noted. Microscopy analysis made possible to note variation between the three wild cat’s fibroblasts. L. colocolo cells are fusiform and presents several cytoplasmic projections common to fibroblasts. L. tigrinus cells are more spherical with short ramifications. P onca fibroblasts are fusiform with longer ramifications. Using Transmission Electron Microscopy, it is noted that digestive vesicles, vacuoles and secretion granules are common, an indicative of intense cellular activity. In relation to cryopreservation, there were no significative differences between the concentrations of, 2.5%, 5% and 10% of DMSO for the L. coloclo and L. tigrinus, both concentrations being efficient in cryoprotecting the fibroblasts from these species. On the other hand, P. onca fibroblasts, were better preserved with solutions contaning 2.5% and 10% DMSO. CryoSOfree™ was efficient to cryopreserve L. colocolo and L. tigrinus fibroblasts. Protocol enhancement will provide the necessary conditions to reproduction for species of interest both on local native fauna and worldwide fauna. Academic studies are also directly enhanced by optimization of such protocols.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB)
Description:	Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia Animal, 2018.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Biologia Animal
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Appears in Collections:	Teses, dissertações e produtos pós-doutorado

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