Título:	Nanopartículas poliméricas contendo clorambucil funcionalizadas com peptídeo direcionador : produção, caracterização e ensaios biológicos com células tumorais
Autor(es):	Dias, Diego Juscelino Santos
Orientador(es):	Gomes, Anderson de Jesus
Coorientador(es):	Gomes, Claure Nain Lunardi
Assunto:	Mamas - câncer Peptídeos Biodisponibilidade Nanopartículas Polímeros Eficiência terapêutica
Data de publicação:	20-Fev-2018
Data de defesa:	28-Ago-2017
Referência:	DIAS, Diego Juscelino Santos. Nanopartículas poliméricas contendo clorambucil funcionalizadas com peptídeo direcionador: produção, caracterização e ensaios biológicos com células tumorais. 2017. xvii, 128 f., il. Tese (Doutorado em Nanociência e Nanobiotecnologia)—Universidade de Brasília, Brasília, 2017.
Abstract:	The breast cancer is a disease that causes fear and death among women. In order to improve the quality of life of patients and to increase the efficiency of treatments already employed clinically, new formulations for the transport and sustained release of a drug were studied. Polymorphic particles containing the chlorambucil antitumor were submitted to two different procedures for primary functionalization (EDC/NHS coupling and DOPA association) and subsequent secondary functionalization with the cyclo-RGD targeting peptide. The obtained samples were evaluated for morphological, cytotoxic and physicochemical characteristics (hydrodynamic diameter and size distribution, zeta potential, in vitro release profile, encapsulation efficiency, thermogravimetric and thermal analysis, spectroscopic analysis in the UV-vis region, FTIR and fluorescence emission). SEM images showed that the samples had spherical shape, low porosity and, considering the data obtained by DLS, presented a size between 290 and 370 nm and PDI between 0,200 and 0,250. Negative values of zeta potential (-10.77 mV) demonstrate that the particles in aqueous medium have low tendency to aggregate. High values of encapsulation efficiency were obtained (mean of 88.0%). Cytotoxicity studies via MTT viability assays and in vitro particle internalization with breast tumor cells (MCF- 7) and fibroblast cells (NIH-3T3) demonstrated that the tripeptide functionalized samples were more efficient in the treatment when compared to control samples or without functionalization. Through the carried out tests, it was possible to conclude that the produced samples are promising systems for transport and sustained release and they could contribute to the increase of selectivity and therapeutic efficiency indexes, bioavailability of site-specificity and consequent reduction of adverse effects of this drug.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB)
Informações adicionais:	Tese (Doutorado)—Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Nanociência e Nanobiotecnologia, 2017.
Programa de pós-graduação:	Programa de Pós-Graduação em Nanociência e Nanobiotecnologia
Licença:	Nanopartículas
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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