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Title: MicroRNA expression profiles in human CD3+ T cells following stimulation with anti‑human CD3 antibodies
Authors: Sousa, Isabel Garcia
Almo, Manuela Maragno do
Simi, Kelly Cristina Rodrigues
Bezerra, Maryani Andressa Gomes
Andrade, Rosângela Vieira
Maranhão, Andréa Queiroz
Brígido, Marcelo de Macedo
Assunto:: Anticorpos
Issue Date: 14-Mar-2017
Publisher: BioMed Central
Citation: SOUSA, Isabel Garcia et al. MicroRNA expression profiles in human CD3+ T cells following stimulation with anti‑human CD3 antibodies. BMC Res Notes, v. 10, Article 124, 14 mar. 2017. Disponível em: <>. Acesso em: 27 jun. 2017. doi:
Abstract: Background: Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays. Results: Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR- 301a) were statistically significantly up- or down-regulated relative to untreated cells. Conclusions: Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
Licença:: © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Appears in Collections:CEL - Artigos publicados em periódicos

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