Título:	Efeito da fonte protéica na produção, qualidade e crioresistência de embriões bovinos produzidos in vitro
Outros títulos:	Effect of protein source in the production, quality and cryoresistance of bovine embryos produced in vitro
Autor(es):	Sena Netto, Severino Bernardino de
Orientador(es):	Pivato, Ivo
Assunto:	Reprodução in vitro Criopreservação de órgãos, tecidos, etc. Embrião Proteínas Bovino Albumina
Data de publicação:	27-Abr-2016
Data de defesa:	5-Fev-2016
Referência:	SENA NETTO, Severino Bernardino de. Efeito da fonte protéica na produção, qualidade e crioresistência de embriões bovinos produzidos in vitro. 2016. xviii, 71 f., il. Dissertação (Mestrado em Ciências Animais)—Universidade de Brasília, Brasília, 2016.
Abstract:	One of the most limiting factors in vitro embryos (IVP) is cryopreservation, because besides the embryos are more sensitive to cold they support better vitrification than slow freezing. But vitrification requires the presence of a technical expert for embryo manipulation while slow freezing allows direct transfer. The objective of this study was to evaluate the effect of replacing SFB by BSA during maturation and embryo culture in response to cryopreservation using slow freezing. The COCs obtained from slaughterhouse ovaries were divided into 2 groups: 1) control: COCs matured and grown in media supplemented with SFB, 2) COCs matured and grown in media supplemented with BSA. D7 in the embryos of each group were divided into two treatments, fresh and frozen. Experiment 1 evaluated the effect of protein source in IVP and response to cryopreservation. After thawing the embryos were evaluated at 24 and 48 hours as the expansion and hatching. In experiment 2 was quantified the expression of genes KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 by qPCR and for the total number of cells and apoptotic cells. The χ2 test and the number of cells performed the data relating to embryonic development and cell apoptotic rate by ANOVA and Tukey test. Cleavage rate was similar (P> 0.05) between groups, however blastocyst rate was higher (P <0.05) in group 1 (42.8 ± 10.1%) than in group 2 (27.9 ± 8.5%). At 24 hours post-thaw SFB group Fresh showed the highest hatching rate (P <0.05) than the others. However, the 48 hours, the hatching rate was similar (P> 0.05) between SFB Control groups (68.1 ± 23.3%) and BSA control (70.0 ± 31.0%) and Frozen SFB (39.2 ± 27.1) and BSA Frozen (38.2 ± 23.9). There was no difference (P> 0.05) between groups Fresh, frozen and between groups regarding the total number of cells. The percentage of apoptotic cells was higher in the Frozen BSA group than group SFB Fresh, did not differ (P <0.05) from the other groups. The KRT8 gene expression was higher (P <0.05) in Frozen FBS group than in BSA Frozen, but was similar (P> 0.05) between frozen and fresh within each group. The PLAC8 gene was more expressed in the BSA Fresh group than in the Frozen SFB group (P <0.05). It can be concluded that the use of BSA replacing FBS caused a reduction in embryo production, but had similar response in cryopreservation. The differential expression of KRT8 and PLAC8 genes suggests that embryos cultured in BSA are top quality.
Unidade Acadêmica:	Faculdade de Agronomia e Medicina Veterinária (FAV)
Informações adicionais:	Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2016.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências Animais
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
DOI:	http://dx.doi.org/10.26512/2016.02.D.20024
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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