Título:	Influência da pentoxifilina na infecção pelo Plasmodium berghei ANKA em camundongos susceptíveis ou resistentes às formas graves da malária : aspectos histopatológicos
Autor(es):	Corazza, Danilo
Orientador(es):	Junqueira, Maria Imaculada Muniz Barboza
Assunto:	Malária Imunologia Histopatologia Pentoxifilina
Data de publicação:	20-Jul-2015
Data de defesa:	12-Fev-2015
Referência:	CORAZZA, Danilo. Influência da pentoxifilina na infecção pelo Plasmodium berghei ANKA em camundongos susceptíveis ou resistentes às formas graves da malária: aspectos histopatológicos. 2015. xxx, 139 f., il. Dissertação (Mestrado em Medicina Tropical)—Universidade de Brasília, Brasília, 2015.
Abstract:	Malaria is still a major problem for global public health, with billions of people in contamination risk areas, and millions affected by the disease, leading to around nearly one million deaths annually. It is an acute febrile illness and in severe forms can present prostration, altered state of consciousness, seizures and pulmonary edema. Pathological manifestations, as febrile paroxysm, cerebral malaria and shock, are due to the increased inflammatory response, with consequent increase in cell adhesion and sequestration of erythrocytes mainly in the endothelium of post-capillary venules. Pentoxifylline (PTX) is a drug with immunomodulatory capacity, regulating the production of inflammatory cytokines, such as tumor necrosis factor (TNF), and with a potential use to prevent severe forms of malaria by reducing the production of TNF. This study evaluated the influence of PTX on the histopathological changes in the brain, liver and lung in mice susceptible or not to severe forms of malaria. C57BL/6, CBA and BALB/c male mice (8 weeks old) were studied. They comprised four groups, in which two were infected with 106 Plasmodium berghei ANKA parasitized erythrocytes and treated with PTX (8 mg/kg/day) or 0.9% NaCl from the third day after infection onward. The two other remaining groups were not infected, but followed the same treatment, with PTX or 0.9% NaCl. On the sixth day after infection, mice were sacrificed and underwent to a cardiac perfusion, the organs were removed (brain, liver and lung) and carried to a histological processing. The slides were stained with hematoxylin-eosin (HE) and evaluated by light microscopy. We observed that the PTX was able to decrease neuronal necrosis in CBA and BALB/c mice. However, the opposite was observed in C57BL/6 mice, in which PTX increased the neuronal necrosis in brain. We also observed that in the cerebral cortex, PTX increased vascular congestion in C57BL/6 model, but decreased vascular congestion in CBA and BALB/c mice. In the liver of the C57BL/6 and BALB/c mice of infected animals that were treated with PTX, it was observed fewer Kupffer cells showing malaria pigment. In lungs of C57BL/6 mice, there was an increase in the permeability of the alveolar-capillary membrane, which was also observed in CBA mice after Plasmodium infection. However, after treatment with PTX, there was a reduction in vascular congestion. Our data suggest that the three strains of mice have different mechanisms of response in each organ, showing the heterogeneity of drug influence, and highlighting the need to better understanding of the ways by which the drug acts in each organ from each of these experimental malaria models.
Unidade Acadêmica:	Faculdade de Medicina (FM)
Informações adicionais:	Dissertação (mestrado)—Universidade de Brasília, Faculdade de Medicina, Programa de Pós-Graduação em Medicina Tropical, 2015.
Programa de pós-graduação:	Programa de Pós-Graduação em Medicina Tropical
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
DOI:	http://dx.doi.org/10.26512/2015.02.D.18461
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