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Title: New vectors derived from pUC18 for cloning and thermal-induced expression in Escherichia coli
Authors: Xavier, Mauro Aparecido de Sousa
Kipnis, André
Torres, Fernando Araripe Gonçalves
Astolfi Filho, Spartaco
Assunto:: Escherichia coli
Clonagem molecular
Genética molecular
Issue Date: Oct-2009
Publisher: Sociedade Brasileira de Microbiologia
Citation: XAVIER, Mauro Aparecido de Sousa et al. New vectors derived from pUC18 for cloning and thermal-induced expression in Escherichia coli. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 4, out./dez. 2009. Disponível em: <>. Acesso em: 27 ago. 2013. DOI:
Abstract: We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
Licença:: Brazilian Journal of Microbiology - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: Acesso em: 27 ago. 2013.
Appears in Collections:CEL - Artigos publicados em periódicos

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