| DC Field | Value | Language |
|---|
| dc.contributor.author | Xavier, Mauro Aparecido de Sousa | - |
| dc.contributor.author | Kipnis, André | - |
| dc.contributor.author | Torres, Fernando Araripe Gonçalves | - |
| dc.contributor.author | Astolfi Filho, Spartaco | - |
| dc.date.accessioned | 2013-08-27T20:41:12Z | - |
| dc.date.available | 2013-08-27T20:41:12Z | - |
| dc.date.issued | 2009-10 | - |
| dc.identifier.citation | XAVIER, Mauro Aparecido de Sousa et al. New vectors derived from pUC18 for cloning and thermal-induced expression in Escherichia coli. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 4, out./dez. 2009. Disponível em: <http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1517-83822009000400007&lng=pt&nrm=iso&tlng=en>. Acesso em: 27 ago. 2013. DOI: http://dx.doi.org/10.1590/S1517-83822009000400007. | en |
| dc.identifier.uri | http://repositorio.unb.br/handle/10482/14041 | - |
| dc.description.abstract | We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7,
for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes
restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of
expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857
repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp
and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple,
non-expensive, high level expression of recombinant proteins in E. coli. | en |
| dc.language.iso | Inglês | en |
| dc.publisher | Sociedade Brasileira de Microbiologia | en |
| dc.rights | Acesso Aberto | en |
| dc.title | New vectors derived from pUC18 for cloning and thermal-induced expression in Escherichia coli | en |
| dc.type | Artigo | en |
| dc.subject.keyword | Escherichia coli | en |
| dc.subject.keyword | Clonagem molecular | en |
| dc.subject.keyword | Genética molecular | en |
| dc.rights.license | Brazilian Journal of Microbiology - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382&lng=pt&nrm=iso. Acesso em: 27 ago. 2013. | en |
| dc.identifier.doi | http://dx.doi.org/10.1590/S1517-83822009000400007 | en |
| Appears in Collections: | Artigos publicados em periódicos e afins
|
