Title:	Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains
Authors:	Silveira, Fabiane Q. de Paula Ximenes, F. A. Cacais, André O. Guerreiro Milagres, A. M. F. Medeiros, C. L. Puls, Jurgen Ferreira Filho, Edivaldo Ximenes
Assunto::	Trichoderma harzianum Xilanase
Issue Date:	1999
Publisher:	Associação Brasileira de Divulgação Científica
Citation:	SILVEIRA, F. Q. P. et al. Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains. Brazilian Journal of Medical and Biological Research, v. 32, n. 8, p. 947-952, 1999. DOI: https://doi.org/10.1590/S0100-879X1999000800003. Disponível em: https://www.scielo.br/j/bjmbr/a/t4CHsgS34S8ksBtyQ8mjwfH/?lang=en#. Acesso em: 10 nov. 2021.
Abstract:	Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and xylopentaose. However, the enzyme was not able to degrade xylohexaose. Xylanase pretreatment was effective for hardwood kraft pulp bleaching. Hardwood kraft pulp bleached in the XEOP sequence had its kappa number reduced from 13.2 to 8.9 and a viscosity of 20.45 cp. The efficiency of delignification was 33%.
Licença::	Brazilian Journal of Medical and Biological Research - This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY). Fonte: https://www.scielo.br/j/bjmbr/a/t4CHsgS34S8ksBtyQ8mjwfH/?lang=en#. Acesso em: 10 nov. 2021.
DOI:	https://dx.doi.org/10.1590/S0100-879X1999000800003
Appears in Collections:	Artigos publicados em periódicos e afins

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