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dc.contributor.authorPlácido, Alexandra-
dc.contributor.authorSilva, Frederico Ferreira da-
dc.contributor.authorLeite, José Roberto de Souza de Almeida-
dc.contributor.authorSantos Álvarez, Noemí de los-
dc.contributor.authorDelerue Matos, Cristina-
dc.date.accessioned2024-03-19T15:36:56Z-
dc.date.available2024-03-19T15:36:56Z-
dc.date.issued2023-02-26-
dc.identifier.citationPLÁCIDO, Alexandra et al. A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed. Plos One, [S.l.], 26 fev. 2020. DOI: https://doi.org/10.1371/journal.pone.0229659. Disponível em: https://doi.org/10.1371/journal.pone.0229659. Acesso em: 19 mar. 2024.pt_BR
dc.identifier.urihttp://repositorio2.unb.br/jspui/handle/10482/48005-
dc.language.isoengpt_BR
dc.publisherPlos Onept_BR
dc.rightsAcesso Abertopt_BR
dc.titleA convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feedpt_BR
dc.typeArtigopt_BR
dc.subject.keywordAlimentos geneticamente modificadospt_BR
dc.subject.keywordAlimentaçãopt_BR
dc.subject.keywordAlimentação dos animaispt_BR
dc.rights.license© 2020 Plácido et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0229659pt_BR
dc.description.abstract1The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commer cial products. To deal with the increased number of GMO events and to address related reg ulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific sam ples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The repro ducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2706-7777pt_BR
dc.contributor.affiliationInstituto Politécnico do Porto, Instituto Superior de Engenharia do Porto, REQUIMTE/LAQVpt_BR
dc.contributor.affiliationUniversidade do Porto, i3S, Instituto de Investigação e Inovação em Saúdept_BR
dc.contributor.affiliationUniversidade do Porto, Instituto de Biologia Molecular e Celularpt_BR
dc.contributor.affiliationUniversidade de Brasília, Campus Darcy Ribeiro, Faculdade de Medicina, Área Morfologiapt_BR
dc.contributor.affiliationUniversidad de Oviedo, Departamento de Química Física y Analíticapt_BR
dc.description.unidadeFaculdade de Medicina (FM)pt_BR
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