Título:	Photodynamic therapy directed to melanoma skin cancer by thermosensitive hydrogel containing Chlorophyll A
Autor(es):	Araújo, Joabe Lima Silva, Patrícia Bento da Santos, Bruno Fonseca Báo, Sônia Nair Chorilli, Marlus Souza, Paulo Eduardo Narcizo de Muehlmann, Luis Alexandre Azevedo, Ricardo Bentes
Afiliação do autor:	University of Brasília, Institute of Biological Sciences, Department of Genetics and Morphology University of Brasília, Institute of Biological Sciences, Department of Genetics and Morphology Federal University of Bahia, Health Sciences Institute, Department of Biotechnology University of Brasília, Institute of Biological Sciences, Cellular Biology Department São Paulo State University, School of Pharmaceutical Sciences, Araraquara University of Brasília, Institute of Physics University of Brasilia, Faculty of Ceilandia University of Brasília, Institute of Biological Sciences, Department of Genetics and Morphology
Assunto:	Terapia fotodinâmica Melanoma Pele - câncer Hidrogel
Data de publicação:	29-Nov-2023
Editora:	MDPI
Referência:	ARAÚJO, Joabe Lima et al. Photodynamic therapy directed to melanoma skin cancer by thermosensitive hydrogel containing Chlorophyll A. Pharmaceuticals, [S. l.], v. 16, n. 12, 1659, 2023. DOI: https://doi.org/10.3390/ph16121659.
Abstract:	Melanoma, a severe form of skin cancer intricately linked to genetic and environmental factors, is predicted to reach 100,000 new cases worldwide by 2040, underscoring the need for effective and safe treatment options. In this study, we assessed the efficacy of a photosensitizer called Chlorophyll A (Chl-A) incorporated into hydrogels (HGs) made of chitosan (CS) and poloxamer 407 (P407) for Photodynamic Therapy (PDT) against the murine melanoma cell line B16-F10. The HG was evaluated through various tests, including rheological studies, SEM, and ATR-FTIR, along with cell viability assays. The CS- and P407-based HGs effectively released Chl-A and possessed the necessary properties for topical application. The photodynamic activity of the HG containing Chl-A was evaluated in vitro, demonstrating high therapeutic potential, with an IC50 of 25.99 µM—an appealing result when compared to studies in the literature reporting an IC50 of 173.8 µM for cisplatin, used as a positive control drug. The developed formulation of CS and P407-based HG, serving as a thermosensitive system for topical applications, successfully controlled the release of Chl-A. In vitro cell studies associated with PDT exhibited potential against the melanoma cell line.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Genética e Morfologia (IB GEM) Departamento de Biologia Celular (IB CEL) Instituto de Física (IF) Faculdade UnB Ceilândia (FCE) Colegiado de Bases Biológicas e da Saúde (FCE-BASES)
Licença:	Copyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/ph16121659
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