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ARTIGO_EngineeredFluorescentStrains.pdf3,98 MBAdobe PDFVisualizar/Abrir
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dc.contributor.authorCastro, Raffael Júnio Araújo de-
dc.contributor.authorRêgo, Marco Túlio Aidar Mariano-
dc.contributor.authorBrandão, Fabiana S.-
dc.contributor.authorAlfonso Pérez, Ana Laura-
dc.contributor.authorDe Marco, Janice Lisboa-
dc.contributor.authorFonseca, Marcio José Poças-
dc.contributor.authorNichols, Connie-
dc.contributor.authorAlspaugh, James Andrew-
dc.contributor.authorFelipe, Maria Sueli Soares-
dc.contributor.authorAlanio, Alexandre-
dc.contributor.authorBocca, Anamélia Lorenzetti-
dc.contributor.authorFernandes, Larissa-
dc.date.accessioned2022-11-28T22:16:51Z-
dc.date.available2022-11-28T22:16:51Z-
dc.date.issued2022-08-25-
dc.identifier.citationCASTRO, Raffael Júnio Araújo de et. al. Engineered fluorescent strains of cryptococcus neoformans: a versatile toolbox for studies of host-pathogen interactions and fungal biology, including the viable but nonculturable state. Microbiology Spectrum, v. 10, n. 5, out. 2022. DOI 10.1128/spectrum.01504-22. Disponível em: https://journals.asm.org/doi/10.1128/spectrum.01504-22. Acesso em: 28 nov. 2022.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/45218-
dc.language.isoInglêspt_BR
dc.rightsAcesso Abertopt_BR
dc.titleEngineered fluorescent strains of cryptococcus neoformans : a versatile toolbox for studies of host-pathogen interactions and fungal biology, including the viable but nonculturable statept_BR
dc.typeArtigopt_BR
dc.subject.keywordCryptococcus neoformanspt_BR
dc.subject.keywordFungos patogênicospt_BR
dc.subject.keywordCepas fluorescentespt_BR
dc.rights.licenseCopyright © 2022 de Castro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Fonte: https://journals.asm.org/doi/pdf/10.1128/spectrum.01504-22. Acesso em: 28 nov. 2022.pt_BR
dc.identifier.doihttps://doi.org/10.1128/spectrum.01504-22pt_BR
dc.description.abstract1Cryptococcus neoformans is an opportunistic fungal pathogen known for its remarkable ability to infect and subvert phagocytes. This ability provides survival and persistence within the host and relies on phenotypic plasticity. The viable but nonculturable (VBNC) phenotype was recently described in C. neoformans, whose study is promising in understanding the pathophysiology of cryptococcosis. The use of fluorescent strains is improving host interaction research, but it is still underexploited. Here, we fused histone H3 or the poly(A) binding protein (Pab) to enhanced green fluorescent protein (eGFP) or mCherry, obtaining a set of C. neoformans transformants with different colors, patterns of fluorescence, and selective markers (hygromycin B resistance [Hygr ] or neomycin resistance [Neor]). We validated their similarity to the parental strain in the stress response, the expression of virulence-related phenotypes, mating, virulence in Galleria mellonella, and survival within murine macrophages. PAB-GFP, the brightest transformant, was successfully applied for the analysis of phagocytosis by flow cytometry and fluorescence microscopy. Moreover, we demonstrated that an engineered fluorescent strain of C. neoformans was able to generate VBNC cells. GFP-tagged Pab1, a key regulator of the stress response, evidenced nuclear retention of Pab1 and the assembly of cytoplasmic stress granules, unveiling posttranscriptional mechanisms associated with dormant C. neoformans cells. Our results support that the PAB-GFP strain is a useful tool for research on C. neoformans.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5207-8892pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3009-627Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9726-3082pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3323-5300pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2814-7501pt_BR
dc.contributor.emailmailto:larissaf@unb.brpt_BR
dc.description.unidadeFaculdade UnB Ceilândia (FCE)-
dc.description.unidadeColegiado de Bases Biológicas e da Saúde (FCE-BASES)-
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