Efects of diferent photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress : a pilot in vitro study

Monteiro, Mylene Martins; Santos, Juliana Amorim dos; Barbosa, Victor Paiva; Gallo, Camila de Barros; Marques, Márcia Martins; Guerra, Eliete Neves Silva

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Campo DC	Valor	Idioma
dc.contributor.author	Monteiro, Mylene Martins	-
dc.contributor.author	Santos, Juliana Amorim dos	-
dc.contributor.author	Barbosa, Victor Paiva	-
dc.contributor.author	Gallo, Camila de Barros	-
dc.contributor.author	Marques, Márcia Martins	-
dc.contributor.author	Guerra, Eliete Neves Silva	-
dc.date.accessioned	2022-08-02T14:28:51Z	-
dc.date.available	2022-08-02T14:28:51Z	-
dc.date.issued	2022-06-07	-
dc.identifier.citation	MONTEIRO, Mylene Martins et al. Efects of diferent photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress: a pilot in vitro study. Lasers in Dental Science, 07 jun. 2022. DOI: https://doi.org/10.1007/s41547-022-00162-1.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/44371	-
dc.language.iso	Inglês	pt_BR
dc.publisher	Springer	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	Efects of diferent photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress : a pilot in vitro study	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Células - cultura e meios de cultura	pt_BR
dc.subject.keyword	Lasers em odontologia	pt_BR
dc.subject.keyword	Fibroblastos	pt_BR
dc.subject.keyword	Fotobiomodulação	pt_BR
dc.identifier.doi	https://doi.org/10.1007/s41547-022-00162-1	pt_BR
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s41547-022-00162-1	pt_BR
dc.description.abstract1	Purpose Photobiomodulation therapy has proven to be effective in accelerating cell proliferation, migration, and transcription. The study aimed to analyze the cell viability effects of different parameters of PBMT in a cultured cell line of human gingival fibroblasts after bacterial and ionizing radiation–induced stress. Methods Explant technique was used to produce a primary cell culture. Cells were grown in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum until stressful condition induction with lipopolysaccharide of Escherichia coli, Porphyromonas gingivalis protein extract, and ionizing radiation. Laser irradiation was carried out in four sessions set with 660 nm wavelength, an output power of 30 mW and 40 mW and energy density of 2, 3, 4, and 5 J/cm2. Results After 24 h from the last laser irradiation session, the groups outputted in 30 mW of power maintained the cell viability while operating with 2, 4, and 5 J/cm2. However, 3 J/cm2 dose significantly decreased cell viability (p < 0.05). When the laser irradiation session was set in a higher power (40 mW), cell viability was reduced using 2, 3, and 5 J/cm2 doses, with statistical significance for 5 J/cm2 (p < 0.001). In addition, operating the same energy using lower power seems to be superior to a higher power, being statically significant for 5 J/cm2 dose (p < 0.001). This pattern followed with all different groups, except by 3 J/cm2. Conclusions The present study showed that delivering 2, 4, and 5 J/ cm2 of density of energy with 30 mW and more time of exposure presented better results on cell viability compared to the same density of energy with output power of 40 mW. Further studies comparing density energy should be conducted.	pt_BR
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