Título:	Molecular detection of piroplasmids in synanthropic rodents, marsupials, and associated ticks from Brazil, with phylogenetic inference of a putative novel Babesia sp. from white-eared opossum (Didelphis albiventris)
Autor(es):	Gonçalves, Luiz Ricardo Paludo, Giane Regina Bisol, Talita Barcelos Perles, Lívia Oliveira, Laryssa Borges de Oliveira, Camila Manoel de Silva, Thiago Merighi Vieira da Nantes, Wesley Arruda Gimenes Duarte, Matheus Almeida Santos, Filipe Martins Porfírio, Grasiela Edith de Oliveira Hirano, Líria Queiroz Luz Herrera, Heitor Miraglia Battesti, Darci Moraes Barros Machado, Rosangela Zacarias André, Marcos Rogério
Assunto:	Capivara Gambá Piroplasmídeos
Data de publicação:	2021
Editora:	Springer Nature
Referência:	GONÇALVES, Luiz Ricardo et al. Molecular detection of piroplasmids in synanthropic rodents, marsupials, and associated ticks from Brazil, with phylogenetic inference of a putative novel Babesia sp. from white-eared opossum (Didelphis albiventris). Parasitology Research, v. 120, n. 10, p. 3537-3546, 2021. DOI: https://doi.org/10.1007/s00436-021-07284-8. Disponível em: https://link.springer.com/article/10.1007/s00436-021-07284-8. Acesso em: 19 maio 2022.
Abstract:	The order Piroplasmida encompasses tick-borne pathogens of veterinary and medical importance positioned in two main families: Babesiidae and Theileriidae. Even though previous studies carried out in Brazil recorded the occurrence of piroplasmid species circulating in small mammals, 18S RNA gene sequences were only partially sequenced, preventing the assessment of their phylogenetic positioning. The current study aimed to detect and characterize, using morphological, molecular, and bioinformatic approaches, piroplasmids from wild mammals and associated ticks sampled in Central-Western Brazil. Out of 67 Didelphis albiventris sampled, 22 (16.4%) were positive for piroplasmids by PCR. In contrast, none of the 48 small rodents and 14 capybaras (Hydrochoerus hydrochaeris) was PCR-positive. Four Amblyomma dubitatum ticks—one from Rattus rattus, one from H. hydrochaeris, and two from D. albiventris—out of 114 Amblyomma spp. DNA samples were positive for piroplasmids by PCR. The phylogenetic inference performed using the near-complete 18S rRNA gene positioned the putative novel piroplasmid species detected in D. albiventris and associated A. dubitatum ticks near to Babesia sensu lato clade (Western group—cluster III) and distant from the Australian marsupial-associated piroplasms. Phylogenetic inferences based on two additional molecular markers, namely hsp-70 and cox-1, supported the near-complete 18S rRNA gene phylogenetic inference. Finally, the partial 18S rRNA gene sequences detected in ticks from rodents (R. rattus and H. hydrochaeris) showed 97.2–99.4% identity with the Piroplasmida previously detected in a capybara from Brazil, raising evidence that a still uncharacterized piroplasmid species has been identified in the capybara, the largest rodent species from South America.
Unidade Acadêmica:	Faculdade de Agronomia e Medicina Veterinária (FAV)
Licença:	© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021
DOI:	https://doi.org/10.1007/s00436-021-07284-8
Versão da editora:	https://link.springer.com/article/10.1007/s00436-021-07284-8
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