Título:	Skin regenerative potential of Cupuaçu seed extract (Theobroma grandiflorum), a native fruit from the Amazon : development of a topical formulation based on chitosan-coated nanocapsules
Autor(es):	Barbalho, Geisa Nascimento Matos, Breno Noronha Brito, Gabriel Ferreira da Silva Miranda, Thamires da Cunha Alencar-Silva, Thuany Fabriz Sodré, Fernando Gelfuso, Guilherme Martins Cunha Filho, Marcílio Sérgio Soares da Carvalho, Juliana Lott de Silva, Joyce Kelly do Rosário da Gratieri, Taís
ORCID:	https://orcid.org/ 0000-0002-9605-4171 https://orcid.org/ 0000-0002-1924-7885 https://orcid.org/ 0000-0002-9167-6852 https://orcid.org/ 0000-0001-6481-2364
Assunto:	Nanocápsulas Extrato de semente de cupuaçu Pele - queimaduras Pele - regeneração Ácido oléico
Data de publicação:	16-Jan-2022
Editora:	MDPI
Referência:	BARBALHO, Geisa Nascimento et al. Skin regenerative potential of Cupuaçu seed extract (Theobroma grandiflorum), a native fruit from the Amazon: development of a topical formulation based on chitosan-coated nanocapsules. Pharmaceutics, v. 14, 207, 2022. DOI: https://doi.org/10.3390/pharmaceutics14010207. Disponível em: https://www.mdpi.com/1999-4923/14/1/207. Acesso em: 26 abr. 2022.
Abstract:	Scarless skin regeneration is a challenge in regenerative medicine. Herein, we explore the regenerative potential of a Cupuaçu seed extract (Theobroma grandiflorum) to develop an innovative skin regeneration formulation based on chitosan-coated nanocapsules. Cupuaçu seed extract significantly stimulated cell proliferation and migration. A reparative gene expression profile could be verified following extract treatment, which included high levels of MKI67, a cellular proliferation marker, and extracellular matrix genes, such as ELN and HAS2, which code for elastin and hyaluronic acid synthase 2. Formulations with Cupuaçu seed extract successfully entrapped into nanocapsules (EE% > 94%) were developed. Uncoated or coated nanocapsules with low-molecular-weight chitosan presented unimodal size distribution with hydrodynamic diameters of 278.3 ± 5.0 nm (PDI = 0.18 ± 0.02) and 337.2 ± 2.1 nm (PDI = 0.27 ± 0.01), respectively. Both nanosystems were physically stable for at least 120 days and showed to be non-irritating to reconstructed human epidermis. Chitosan coating promoted active penetration into undamaged skin areas, which were still covered by the stratum corneum. In conclusion, the present study demonstrated for the first time the biotechnological potential of the frequently discarded Cupuaçu seed as a valuable pharmaceutical ingredient to be used in regenerative skin products.
Unidade Acadêmica:	Faculdade de Medicina (FMD)
Licença:	Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/pharmaceutics14010207
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