Título:	Ramularia leaf spot : PCR-based methods reveal widespread distribution of Ramulariopsis pseudogycines and limited presence of R. gossypii in Brazil
Autor(es):	Silva, Aline Suelen Renno, Marcelo Henrique Lisboa Quitania, Ana Clara Ribeiro Café Filho, Adalberto Correa Miller, Robert Neil Gerard Araujo, Alderi Emidio Pinho, Danilo Batista
Assunto:	Algodão - pragas e doenças Fungos patogênicos
Data de publicação:	2021
Editora:	https://doi.org/10.21203/rs.3.rs-477759/v1
Referência:	SILVA, Aline Suellen et al. Ramularia leaf spot: PCR-based methods reveal widespread distribution of Ramulariopsis pseudogycines and limited presence of R. gossypii in Brazil. Research Square Preprints, 2021. DOI: https://doi.org/10.21203/rs.3.rs-477759/v1. Disponível em: https://www.researchsquare.com/article/rs-477759/v1
Abstract:	Whilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield across all growing regions. In 2017-18 and 2018-19 growing seasons, ca. 300 fungal samples were collected from seven Brazilian states. Hyphal tip cultures were obtained for DNA extraction and amplification of partial sequences of the RNA polymerase II gene (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) gene regions. Genetic diversity of the isolates was evaluated using fourteen molecular markers. Clade assignments based on the concatenated-sequence tree (RPB2, LSU, EF1-α, ITS, ACT, and HIS3) were identical to those in tree clades generated by RPB2-sequences, as well as in an RPB2 haplotype network, in an ISSR (TGTC)4 dendrogram, and based on morphological comparisons. In total, 252 out of 267 isolates were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions today. Validation of the ISSR (TGTC)4 primer as a tool to study the diversity and distribution of Ramulariopsis species will make it possible to carry out extensive RLS sampling studies worldwide.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Fitopatologia (IB FIT)
Informações adicionais:	This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Licença:	This work is licensed under a CC BY 4.0 License.
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