| Campo DC | Valor | Idioma |
|---|
| dc.contributor.author | Oliveira, Viviane C. | - |
| dc.contributor.author | Macedo, Ana P. | - |
| dc.contributor.author | Melo, Luís D. R. | - |
| dc.contributor.author | Santos, Sílvio B. | - |
| dc.contributor.author | Hermann, Paula Regina de Souza | - |
| dc.contributor.author | Silva-Lovato, Cláudia H. | - |
| dc.contributor.author | Paranhos, Helena F. O. | - |
| dc.contributor.author | Andrade, Denise | - |
| dc.contributor.author | Watanabe, Evandro | - |
| dc.date.accessioned | 2021-02-01T14:46:49Z | - |
| dc.date.available | 2021-02-01T14:46:49Z | - |
| dc.date.issued | 2021-01-15 | - |
| dc.identifier.citation | OLIVEIRA, Viviane C. et al. Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface. Antibiotics, v. 10, n. 1, 78, 2021. DOI: https://doi.org/10.3390/antibiotics10010078. Disponível em: https://www.mdpi.com/2079-6382/10/1/78/htm. Acesso em: 01 fev. 2021. | pt_BR |
| dc.identifier.uri | https://repositorio.unb.br/handle/10482/39999 | - |
| dc.language.iso | Inglês | pt_BR |
| dc.publisher | MDPI | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Bacteriófago | pt_BR |
| dc.subject.keyword | Biofilme | pt_BR |
| dc.subject.keyword | Pseudomonas aeruginosa | pt_BR |
| dc.subject.keyword | Tubo endotraqueal | pt_BR |
| dc.rights.license | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | pt_BR |
| dc.identifier.doi | https://doi.org/10.3390/antibiotics10010078 | pt_BR |
| dc.description.abstract1 | Although different strategies to control biofilm formation on endotracheal tubes have
been proposed, there are scarce scientific data on applying phages for both removing and preventing
Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five
bacteriophages was evaluated by a high content screening assay. We observed that biofilms were
significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering
the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail’s ability
to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model.
Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was
monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and
biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization;
nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial
changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of
biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes.
These results demonstrate that with the development of new coating strategies, phage therapy has
potential in controlling the endotracheal tube-associated biofilm. | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0002-0734-0652 | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0002-6865-6044 | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0003-0801-8080 | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0003-1629-2207 | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0001-5674-2589 | pt_BR |
| Aparece nas coleções: | Artigos publicados em periódicos e afins
|
