Título:	Trypanosoma cruzi infection down-modulates the Immunoproteasome Biosynthesis and the MHC class I cell surface expression in HeLa cellsurface expression in HeLa cells
Autor(es):	Camargo, Ricardo Faria, Liliam O. Kloss, Alexander Favali, Cecilia Beatriz Fiuza Kuckelkorn, Ulrike Kloetze, Peter Michael Sá, Cezar Martins de Lima, Beatriz D.
Assunto:	Trypanosoma cruzi Chagas, Doença de
Data de publicação:	21-Abr-2014
Editora:	Plos One
Referência:	CAMARGO, Ricardo et al. Trypanosoma cruzi infection down-modulates the Immunoproteasome Biosynthesis and the MHC class I cell surface expression in HeLa cellsurface expression in HeLa cells. Plos One, v. 9, n. 4, Article e95977, 21 abr. 2014. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095977>. Acesso em: 13 jun. 2017. doi: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095977.
Abstract:	Generally, Trypanosoma cruzi infection in human is persistent and tends to chronicity, suggesting that the parasite evade the immune surveillance by down regulating the intracellular antigen processing routes. Within the MHC class I pathway, the majority of antigenic peptides are generated by the proteasome. However, upon IFN-c stimulation, the catalytic constitutive subunits of the proteasome are replaced by the subunits b1i/LMP2, b2i/MECL-1 and b5i/LMP7 to form the immunoproteasome. In this scenario, we analyzed whether the expression and activity of the constitutive and the immunoproteasome as well as the expression of other components of the MHC class I pathway are altered during the infection of HeLa cells with T. cruzi. By RT-PCR and two-dimensional gel electrophoresis analysis, we showed that the expression and composition of the constitutive proteasome is not affected by the parasite. In contrast, the biosynthesis of the b1i, b2i, b5i immunosubunits, PA28b, TAP1 and the MHC class I molecule as well as the proteasomal proteolytic activities were down-regulated in infected-IFN-c-treated cell cultures. Taken together, our results provide evidence that the protozoan T. cruzi specifically modulates its infection through an unknown posttranscriptional mechanism that inhibits the expression of the MHC class I pathway components.
Licença:	Copyright: 2014 Camargo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095977. Acesso em: 13 jun. 2017.
DOI:	https://dx.doi.org/10.1371/journal.pone.0095977
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