Título:	Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative Pleiotropic Effect on Nematode Vigor
Autor(es):	Souza Júnior, José Dijair Antonino de Coelho, Roberta Ramos Lourenço, Isabela Tristan Fragoso, Rodrigo da Rocha Viana, Antonio Américo Barbosa Macedo, Leonardo Lima Pepino de Silva, Maria Cristina Mattar da Carneiro, Regina Maria Gomes Engler, Gilbert Engler, Janice de Almeida Sá, Maria Fatima Grossi de
Assunto:	Nematóides Proteínas Morfologia Células Enzimas proteolíticas
Data de publicação:	31-Dez-2013
Editora:	Plos One
Referência:	SOUZA JÚNIOR, José Dijair Antonino de et al. Knocking-Down Meloidogyne incognita Proteases by plant-delivered dsRNA has negative Pleiotropic Effect on Nematode Vigor. Plos ONe, v. 8, n. 12, Article: e85364, 31 dez. 2013. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085364>. Acesso em: 12 jun. 2017. doi: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085364.
Abstract:	The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita proteases could play important roles in nematode parasitism, besides their function in ordinary digestion of giant cell contents for feeding. The precise roles of these proteins during parasitism however are still unknown, making them interesting targets for gene silencing to address protein function. In this study we have knocked-down an aspartic (Mi-asp-1), a serine (Mi-ser-1) and a cysteine protease (Mi-cpl-1) by RNAi interference to get an insight into the function of these enzymes during a host/nematode interaction. Tobacco lines expressing dsRNA for Mi-ser-1 (dsSER), Mi-cpl-1 (dsCPL) and for the three genes together (dsFusion) were generated. Histological analysis of galls did not show clear differences in giant cell morphology. Interestingly, nematodes that infected plants expressing dsRNA for proteases produced a reduced number of eggs. In addition, nematode progeny matured in dsSER plants had reduced success in egg hatching, while progeny resulting from dsCPL and dsFusion plants were less successful to infect wild-type host plants. Quantitative PCR analysis confirmed a reduction in transcripts for Mi-cpl-1 and Mi-ser-1 proteases. Our results indicate that these proteases are possibly involved in different processes throughout nematode development, like nutrition, reproduction and embryogenesis. A better understanding of nematode proteases and their possible role during a plant-nematode interaction might help to develop new tools for phytonematode control.
Licença:	Copyright: © 2013 Antonino de Souza Júnior et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI:	https://dx.doi.org/10.1371/journal.pone.0085364
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