Título:	Avaliação da atividade antimicrobiana de nanoemulsões dos óleos essenciais de vetiver (Chrysopogon zizanioides) e aroeira (Schinus terebinthifolius)
Autor(es):	Prado, Bruno Alcântara do
Orientador(es):	Orsi, Daniela Castilho
Assunto:	Óleos essenciais Aroeira Nanoemulsão Atividade antimicrobiana
Data de publicação:	26-Jun-2026
Data de defesa:	16-Dez-2026
Referência:	PRADO, Bruno Alcântara do. Avaliação da atividade antimicrobiana de nanoemulsões dos óleos essenciais de vetiver (Chrysopogon zizanioides) e aroeira (Schinus terebinthifolius). 2026. 115 f. il. Dissertação (Mestrado em Ciências e Tecnologias em Saúde) — Universidade de Brasília, Brasília, 2025.
Abstract:	The antimicrobial activity of essencial oils has been widely investigated as na alternative to enhance the action of antimicrobial agentes and to contribute to the reduction os bacterial resistance. The essencial oils of Chrysopogon zizanioides (vetiver) and Schinus terebinthifolius (aroeira) exhibit a complex Chemical composition, consisting of secondary metabolites with well-established antimicrobial activity. These oils are lipopholic, volatile, and unstable substances, and may lose their biological activity When exposed to Oxygen, light, and variations in temperature and pH. Nanoemulsification emerges as na effective strategy to preserve the active compounds, increase stability, improve bioavailability, and enhance solubility in aqueous media. Therefore, the present study aimed to evaluate the antimicrobial activity of vetiver and aroeira essencial oils and their respective nanoemulsions, as well as to peform the Chemical characterization of the essencial oils. Chemical composition was determined by gas chromatography. The nanoemulsions were formulated and characterized in terms of droplet size, polydispersity index (PDI), and zeta potential. Antimicrobial activity was assessed using the broth microdilution method to determine the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). Time-kill assays were performed with vetiver essential oil and its nanoemulsion against Staphylococcus aureus and Escherichi coli. In vetiver essential oil, the major compounds were vetivenic acid (22,95%), α-vetivene (7,50%), khusimol (7,42%), and β-vetivene (5,63%). In aroeira essential oil, The predominant compounds were α-phellandrene (35,96%), α-pinene (15,41%), limonene (14,39%), and δ-3-carene (10,70%). The nanoemulsions presented mean droplet sizes of 105,7 nm (Brazilian pepper tree) and 304,7 nm (vetiver), with PDI values of 0,20 and 0,32 and zeta potentials of 0,33 mV and -0,82 mV, respectively. Vetiver essential oil exhibited high antimicrobial activity Against Gram-positive bactéria, particularly Bacillus cereus (MIC 0,10 μL/mL) and S. aureus (MIC 0,40 μL/mL), and lower activity Against Gram-negative bactéria. The vetiver nanoemulsion enhanced antimicrobial activity, reducing MIC values for several strains. Similar results were observed for the aroeira oil. In the time-kill assays, the vetiver nanoemulsion demonstrated a faster and more effective bactericidal effect, especially against E.coli. It can be concluded that nanoemulsification representes a viable and effective technological alternative to enhance the antimicrobial activity of vetiver and aroeira
Unidade Acadêmica:	Faculdade de Ciências e Tecnologias em Saúde (FCTS) – Campus UnB Ceilândia
Informações adicionais:	Dissertação (mestrado) — Universidade de Brasília, Faculdade de Ceilândia, Programa de Pós-graduação em Ciências e Tecnologias em Saúde, 2026.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências e Tecnologias em Saúde
Agência financiadora:	Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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