Título:	Sylvatic mosquito viromes in the Cerrado biome of Minas Gerais, Brazil : discovery of new viruses and implications for arbovirus transmission
Autor(es):	Maia, Luis Janssen Silva, Arthur Batista Oliveira, Cirilo Henrique de Campos, Fabricio Souza Silva, Leonardo Assis da Abreu, Filipe Vieira Santos de Ribeiro, Bergmann Morais
ORCID:	https://orcid.org/0000-0002-5772-0477 https://orcid.org/0000-0002-3734-8735 https://orcid.org/0000-0002-5948-472X https://orcid.org/0000-0001-9768-4688 https://orcid.org/0000-0002-1061-196X
Afiliação do autor:	Laboratório de Baculovírus, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília e Laboratório de Bioinformática e Biotecnologia, Universidade Federal do Tocantins Laboratório de Bioinformática e Biotecnologia, Universidade Federal do Tocantins Laboratório de Comportamento de Insetos, Instituto Federal do Norte de Minas Gerais (IFNMG), Programa de Pós-Graduação em Biodiversidade e Uso dos Recursos Naturais, Unimontes e Centro Colaborador de Entomologia/Lacoi/IFNMG Laboratório de Bioinformática e Biotecnologia, Universidade Federal do Tocantins (UFT) e Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS) Laboratório de Baculovírus, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília (UnB) Laboratório de Comportamento de Insetos, Instituto Federal do Norte de Minas Gerais (IFNMG) e Centro Colaborador de Entomologia/Lacoi/IFNMG/Secretaria Municipal de Saúde de Salinas Laboratório de Baculovírus, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília (UnB)
Assunto:	Mosquitos RNA Cerrado Arbovírus Biodiversidade
Data de publicação:	9-Ago-2024
Editora:	MDPI
Referência:	MAIA, Luis Janssen et. al. Sylvatic mosquito viromes in the Cerrado biome of Minas Gerais, Brazil: discovery of new viruses and implications for arbovirus transmission. Viruses, [S. l.], v. 16, 2024. DOI: https://doi.org/10.3390/v16081276. Disponível em: https://www.mdpi.com/1999-4915/16/8/1276. Acesso em: 31 mar. 2026.
Abstract:	Studies on animal virome have mainly concentrated on chordates and medically significant invertebrates, often overlooking sylvatic mosquitoes, constituting a major part of mosquito species diversity. Despite their potential role in arbovirus transmission, the viromes of sylvatic mosquitoes remain largely unexplored. These mosquitoes may also harbor insect-specific viruses (ISVs), affecting arboviral transmission dynamics. The Cerrado biome, known for rapid deforestation and its status as a biodiversity hotspot, offers an ideal setting for investigating mosquito viromes due to potential zoonotic spillover risks from land use changes. This study aimed to characterize the viromes of sylvatic mosquitoes collected from various locations within Minas Gerais state, Brazil. The total RNA was extracted from mosquito pools of Psorophora albipes, Sabethes albiprivus, Sa. chloropterus, Psorophora ferox, and Coquillettidia venezuelensis species, followed by high-throughput sequencing (HTS). Bioinformatic analysis included quality control, contig assembly, and viral detection. Sequencing data analysis revealed 11 near-complete viral genomes (new viruses are indicated with asterisks) across seven viral families and one unassigned genus. These included: Xinmoviridae (Ferox mosquito mononega-like virus* and Albipes mosquito Gordis-like virus*), Phasmaviridae (Sabethes albiprivus phasmavirus*), Lispiviridae (Pedras lispivirus variant MG), Iflaviridae (Sabethes albiprivus iflavivirus*), Virgaviridae (Buriti virga-like virus variant MG and Sabethes albiprivus virgavirus 1*), Flaviviridae (Psorophora ferox flavivirus*), Mesoniviridae (Alphamesonivirus cavallyense variant MG), and the genus Negevirus (Biggie virus variant MG virus and Coquillettidia venezuelensis negevirus*). Moreover, the presence of ISVs and potential novel arboviruses underscores the need for ongoing surveillance and control strategies to mitigate the risk of emerging infectious diseases.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Programa de pós-graduação:	Programa de Pós-Graduação em Biologia Molecular
Licença:	(CC-BY) © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/v16081276
Versão da editora:	https://www.mdpi.com/1999-4915/16/8/1276
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