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dc.contributor.authorAndrade, Kellen Cruvinel Rodrigues-
dc.contributor.authorHomem-de-Mello, Mauricio-
dc.contributor.authorMotta, Julia Almeida-
dc.contributor.authorGuimarães, Marina Borges-
dc.contributor.authorAbreu, Joel Antônio Cordeiro de-
dc.contributor.authorSouza, Paula Monteiro de-
dc.contributor.authorPessoa, Adalberto-
dc.contributor.authorPappas Júnior, Georgios Joannis-
dc.contributor.authorMagalhães, Pérola de Oliveira-
dc.date.accessioned2025-10-28T15:38:32Z-
dc.date.available2025-10-28T15:38:32Z-
dc.date.issued2024-
dc.identifier.citationANDRADE, Kellen Cruvinel Rodrigues et al. A structural In silico analysis of the immunogenicity of L-Asparaginase from Penicillium cerradense. International Journal of Molecular Sciences, v. 25, n. 9, 2024. DOI: https://doi.org/10.3390/ijms25094788. Disponível em: https://www.mdpi.com/1422-0067/25/9/4788. Acesso em: 28 out. 2025.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/52934-
dc.language.isoengpt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleA structural in silico analysis of the immunogenicity of L-Asparaginase from Penicillium cerradensept_BR
dc.typeAnaispt_BR
dc.subject.keywordL-asparaginasept_BR
dc.subject.keywordImunogenicidadept_BR
dc.subject.keywordPenicillium cerradensept_BR
dc.identifier.doihttps://doi.org/10.3390/ijms25094788pt_BR
dc.description.abstract1L-asparaginase is an essential drug used to treat acute lymphoid leukemia (ALL), a cancer of high prevalence in children. Several adverse reactions associated with L-asparaginase have been observed, mainly caused by immunogenicity and allergenicity. Some strategies have been adopted, such as searching for new microorganisms that produce the enzyme and applying protein engineering. Therefore, this work aimed to elucidate the molecular structure and predict the immunogenic profile of L-asparaginase from Penicillium cerradense, recently revealed as a new fungus of the genus Penicillium and producer of the enzyme, as a motivation to search for alternatives to bacterial L-asparaginase. In the evolutionary relationship, L-asparaginase from P. cerradense closely matches Aspergillus species. Using in silico tools, we characterized the enzyme as a protein fragment of 378 amino acids (39 kDa), including a signal peptide containing 17 amino acids, and the isoelectric point at 5.13. The oligomeric state was predicted to be a homotetramer. Also, this L-asparaginase presented a similar immunogenicity response (T- and B-cell epitopes) compared to Escherichia coli and Dickeya chrysanthemi enzymes. These results suggest a potentially useful L-asparaginase, with insights that can drive strategies to improve enzyme production.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4541-9177pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8767-407Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5969-0806pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5268-8690pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1100-976Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8011-6940pt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Productspt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, inSiliToxpt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, inSiliToxpt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Productspt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Productspt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Productspt_BR
dc.contributor.affiliationUniversity of São Paulo, School of Pharmaceutical Sciences, Department of Biochemical and Pharmaceutical Technologypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute Biological Sciences, Department Cell Biologypt_BR
dc.contributor.affiliationUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Productspt_BR
dc.description.unidadeFaculdade de Ciências da Saúde (FS)pt_BR
dc.description.unidadeDepartamento de Farmácia (FS FAR)pt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Biologia Celular (IB CEL)pt_BR
dc.description.ppgPrograma de Pós-Graduação em Ciências Farmacêuticaspt_BR
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