Titre:	Presence of host and bacterial-derived collagenolytic proteases in carious dentin : a systematic review of ex vivo studies
Auteur(s):	Barbosa, Cecília de Brito Silva, Isabela Monici Cena, Jéssica Alves de Stefani, Cristine Miron Teixeira, Nailê Damé
metadata.dc.identifier.orcid:	https://orcid.org/0000-0003-1292-2371 https://orcid.org/0000-0002-4010-4280 https://orcid.org/0000-0001-6442-5639 https://orcid.org/0000-0003-4712-9779 https://orcid.org/0000-0002-0490-0036
metadata.dc.contributor.affiliation:	University of Brasília, School of Health Sciences, Department of Dentistry University of Brasília, School of Health Sciences, Department of Dentistry University of Brasília, School of Health Sciences, Department of Dentistry University of Brasília, School of Health Sciences, Department of Dentistry University of Brasília, School of Health Sciences, Department of Dentistry
Assunto::	Dentes - dentina Cárie dentária Colagenase microbiana
Date de publication:	31-oct-2023
Editeur:	Frontiers
Référence bibliographique:	BARBOSA, Cecília de Brito et al. Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo studies. Frontiers in Cellular and Infection Microbiology, v. 13, art. 1278754, 2023. DOI: DOI 10.3389/fcimb.2023.1278754. Disponível em: https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1278754/full. Acesso em: 03 jul. 2025.
Abstract:	Introduction and aim: The presence of host collagenases in the degradation of the protein matrix at later stages of carious dentin lesions development, as well as the potential involvement of bacterial collagenases, have been suggested but lack conclusive evidence. This study aims to conduct a systematic review to comprehensively assess the profile of host and bacterial-derived collagenolytic proteases in both root and coronal dentin carious lesions. Methods: The search was performed in eight databases and the grey literature. Studies evaluating ex vivo dentin, extracted teeth, or biofilms from natural caries lesions were included. The methodological quality of studies was assessed using the Joanna Briggs Institute tool. Synthesis of the results and the certainty of evidence were performed following the Synthesis without Meta-analysis (SWiM) checklist and GRADE approach for narrative synthesis, respectively. Results: From 935 recovered articles, 18 were included. Although the evidence was very uncertain, it was possible to suggest that 1) MMP-2, MMP-9, MMP-13, and CT-B may be increased in carious dentin when compared to sound dentin; 2) there is no difference in MMP-2 presence, while MMP-13 may be increased in root when compared to coronal carious dentin; 3) there is no difference of MMP2 and MMP-9 expression/activity before and after cavity sealing; 4) MMP-8 may be increased in the dentin before cavity sealing compared to dentin after cavity sealing; 5) there is no difference of MMP-20 in irradiated vs. non-irradiated carious dentin. MMP-20 probably reduces in carious outer dentin when compared to carious inner dentin (moderate certainty). Genes encoding bacterial collagenolytic proteases and protein-degrading bacteria were detected in coronal and root carious lesions. Conclusion: Trends in the direction of the effect were observed for some collagenolytic proteases in carious dentin, which may represent a potential target for the development of new treatments. (Protocol register-PROSPERO: CRD42020213141).
metadata.dc.description.unidade:	Faculdade de Ciências da Saúde (FS) Departamento de Odontologia (FS ODT)
metadata.dc.description.ppg:	Programa de Pós-Graduação em Odontologia
Licença::	© 2023 Barbosa, Monici Silva, Cena, Stefani and Dame-Teixeira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI:	10.3389/fcimb.2023.1278754
Collection(s) :	Artigos publicados em periódicos e afins

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