Título:	Produção e otimização de lacases e Peroxidases obtidas de Diaporthe spp. isolados com endofíticos de Solanum falciforme Farruggia
Outros títulos:	Production and optimization of laccases and peroxidases obtained from Diaporthe spp. isolated as endophytic from Solanum falciforme Farruggia
Autor(es):	Andrade, Jayanaraian Ferreira Martins
Orientador(es):	Mello, Maurício Homem de
Coorientador(es):	Batista, Pérola Magalhães de Oliveira Dias
Assunto:	Diaporthe Enzimas lignolíticas Planejamento fatorial
Data de publicação:	3-Mai-2021
Data de defesa:	14-Jan-2021
Referência:	ANDRADE, Jayanaraian Ferreira Martins. Produção e otimização de lacases e Peroxidases obtidas de Diaporthe spp. isolados com endofíticos de Solanum falciforme Farruggia. 2021. 83 f., il. Dissertação (Mestrado em Ciências Farmacêuticas)—Universidade de Brasília, Brasília, 2021.
Abstract:	Endophytic fungi are microorganisms able to colonize the interior of plant hosts in intercellular spaces or within cells, maintaining a symbiotic relationship. Endophytic fungi can be isolated from several plant species. Their biotechnological potential as enzyme producers has gained more interest in the last decade. In this work, two endophytic fungi were isolated from Solanum falciforme Farruggia, native to Brazilian Cerrado. Isolates, “P1” and “P2” were subjected to molecular identification, using the ITS region to establish their phylogenetic relationship, isolate “P1” was clustered with Diaporthe stictica, while “P2” was clustered with Diaporthe maritima. The isolates were identified as belonging to the genus Diaporthe, reported as one of the most frequently isolated genera of endophytic fungi. They were analyzed for their ability to produce oxidative enzymes: laccases and total peroxidases. Curves of 30 and 15 days were performed to establish the best day of production of these enzymes for each isolate. For “P1”, the 11th day was selected (38.63 Ulaccase/mL; 46.77 Utotal peroxidases/mL) and for “P2” the 10th day (P2: 41.21 Ulaccase/mL; 13.55 Utotal peroxidases/mL). For each isolate a 23 experimental design was carried out with a central point in triplicate to optimize enzymatic production, testing the following factors: initial pH of the medium at levels 4 and 8, amount of peptone, as nitrogen source, at levels 0g and 1g and sugarcane bagasse, as carbon source, at levels 0.25g and 0.75g; central point combined initial pH, peptone and sugarcane bagasse at levels 6, 1.0g and 0.50g, respectively. No model established by the experimental designs obtained statistical significance according to the levels at which these factors were tested (confidence level: 95%), only the factors initial pH, peptone and the interaction between them were considered significant (p <0.05) for the production of laccase for the “P1” isolate. In general, enzyme production increased when the initial pH was at the lowest level and the amount of peptone at the highest level. This work generated relevant data on the extracellular production of oxidative enzymes by endophytic fungi of the genus Diaporthe.
Unidade Acadêmica:	Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
Informações adicionais:	Dissertação (mestrado)—Universidade de Brasília, Faculdade de Ciências da Saúde, Brasília, 2021.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências Farmacêuticas
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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