Título:	Desenvolvimento de nanocápsulas de latanoprosta para o tratamento tópico de alopecia
Autor(es):	Oliveira, Ana Christina Silva de
Orientador(es):	Gelfuso, Guilherme Martins
Assunto:	Alopecia androgênica - tratamento Nanopartículas poliméricas Latanoprosta
Data de publicação:	21-Dez-2020
Data de defesa:	29-Jul-2020
Referência:	OLIVEIRA, Ana Christina Silva de. Desenvolvimento de nanocápsulas de latanoprosta para o tratamento tópico de alopecia. 2020. xiii, 65 f., il. Dissertação (Mestrado em Ciências da Saúde)—Universidade de Brasília, Brasília, 2020.
Abstract:	Alopecia is a disease that affects the hair follicles, gradually decreasing the production of hair shafts. Latanoprost has recently been shown to be a promising molecule for the treatment of this disease, given its influence on hair follicle cells. Considering that the high lipophilicity of this molecule hinders its incorporation in an aqueous-based formulation and restricts its cutaneous penetration, this work aimed to develop a formulation based on polymeric nanocapsules of polycaprolactone incorporating latanoprost in an attempt to promote the accumulation of this substance in hair follicles to favor topical alopecia therapy. The nanocapsules were obtained following the solvent evaporation method. The produced nanosystems had their average size, polydispersity index (PdI) and zeta potential measured through the analysis of light scattering and electrophoretic mobility. The encapsulation efficiency values were determined by quantifying the non-encapsulated latanoprost separated by centrifugation. The stability of the developed nanosystem was monitored for 90 days, observing characteristics such as average size, polydispersity index, zeta potential and drug content. For permeation studies, diffusion cells were assembled with pig ear skin separating the donor compartment from the recipient behavior. Nanocapsules and control formulations were applied for 8 h on the skin surface. The experiments were carried out with and without mechanical stimulation on the skin after the formulation was applied. Nanocapsules of 197.0 ± 2.2 nm (PdI = 0.15 ± 0.01), with zeta potential of -30.1 ± 1.8 mV and capable of encapsulating 93.9 ± 0.4% of latanoprost were obtained. They were stable for at least 90 days when stored at 6oC (209.8 ± 2.31 nm; PdI = 0.35 ± 0.04; zeta potential of -29.2 ± 0.25). Permeation studies showed there was an increase of approximately 20% in the accumulation of latanoprost in hair follicles in the application of nanocapsules’ formulation compared to the control formulation. This difference increased to 86% when the nanocapsules were applied to the skin under a manual massage. Thus, considering that the colloidal dispersion of nanocapsules also demonstrated a very low irritating potential for the skin, it can be concluded that the developed system has a high potential for use as a topical formulation for the treatment of androgenic alopecia.
Unidade Acadêmica:	Faculdade de Ciências da Saúde (FS)
Informações adicionais:	Dissertação (mestrado)—Universidade de Brasília, Faculdade de Ciências da Saúde, Programa de Pós-Graduação em Ciências da Saúde, 2020.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências da Saúde
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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