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Title: Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®
Authors: Almeida, Simoneide Souza Titze de
Horst, Camila Hillesheim
Sánchez, Cristina Soto
Fernandez, Eduardo
Almeida, Ricardo Titze de
metadata.dc.identifier.orcid: https://orcid.org/0000-0001-7286-1676
Assunto:: MicroRNA
Nanopartículas
Neuromag®
Issue Date: 23-Jul-2018
Publisher: MDPI
Citation: ALMEIDA, Simoneide Souza Titze de et al. Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®. Molecules, v. 23, n. 7, 1825, 2018. DOI: https://doi.org/10.3390/molecules23071825. Disponível em: https://www.mdpi.com/1420-3049/23/7/1825. Acesso em: 24 nov. 2020.
Abstract: MicroRNAs (miRNAs) regulate gene expression at posttranscriptional level by triggering RNA interference. In such a sense, aberrant expressions of miRNAs play critical roles in the pathogenesis of many disorders, including Parkinson’s disease (PD). Controlling the level of specific miRNAs in the brain is thus a promising therapeutic strategy for neuroprotection. A fundamental need for miRNA regulation (either replacing or inhibition) is a carrier capable of delivering oligonucleotides into brain cells. This study aimed to examine a polymeric magnetic particle, Neuromag®, for delivery of synthetic miRNA inhibitors in the rat central nervous system. We injected the miRNA inhibitor complexed with Neuromag® into the lateral ventricles next to the striatum, by stereotaxic surgery. Neuromag efficiently delivered oligonucleotides in the striatum and septum areas, as shown by microscopy imaging of fluorescein isothiocyanate (FITC)-labeled oligos in astrocytes and neurons. Transfected oligos showed efficacy concerning miRNA inhibition. Neuromag®-structured miR-134 antimiR (0.36 nmol) caused a significant 0.35 fold decrease of striatal miR-134, as revealed by real-time quantitative polymerase chain reaction (RT-qPCR). In conclusion, the polymeric magnetic particle Neuromag® efficiently delivered functional miRNA inhibitors in brain regions surrounding lateral ventricles, particularly the striatum. This delivery system holds potential as a promising miRNA-based disease-modifying drug and merits further pre-clinical studies using animal models of PD.
Licença:: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI: https://doi.org/10.3390/molecules23071825
Appears in Collections:FAV - Artigos publicados em periódicos

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