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Title: Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract
Authors: Tormena, Renata Pascoal Illanes
Rosa, Eliane Vieira
Mota, Bruna de Fatima Oliveira
Chaker, Juliano Alexandre
Fagg, Christopher William
Freire, Daniel Oliveira
Martins, Paula Melo
Silva, Izabel Cristina Rodrigues da
Sousa, Marcelo Henrique
metadata.dc.identifier.orcid: https://orcid.org/0000-0003-3248-1286
https://orcid.org/0000-0002-2144-0959
https://orcid.org/0000-0001-6072-7832
https://orcid.org/0000-0003-1210-8329
Assunto:: Atividade antimicrobiana
Nanopartículas de prata
Ipê-roxo
Issue Date: 1-Jun-2020
Publisher: The Royal Society of Chemistry
Citation: TORMENA, Renata Pascoal Illanes et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, v. 10, 20676-20681, 2020. DOI: https://doi.org/10.1039/D0RA03240A. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A#!divAbstract. Acesso em: 10 nov. 2020.
Abstract: We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 °C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline ∼13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains.
Licença:: Open Access Article - (CC BY-NC) - This article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence.
DOI: https://doi.org/10.1039/D0RA03240A
Appears in Collections:FCE-FAR - Artigos publicados em periódicos
TEC UnB - Artigos publicados em periódicos

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