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Title: Phylloseptin-1 is leishmanicidal for amastigotes of Leishmania amazonensis inside infected macrophages
Authors: Kückelhaus, Selma Aparecida Souza
Aquino, Daniela Sant’Ana de
Borges, Tatiana Karla dos Santos
Moreira, Daniel Carneiro
Leite, Luciana de Magalhães
Junqueira, Maria Imaculada Muniz Barboza
Kückelhaus, Carlos S.
Romero, Gustavo Adolfo Sierra
Prates, Maura Vianna
Bloch Júnior, Carlos
Leite, José Roberto de Souza de Almeida
metadata.dc.identifier.orcid: https://orcid.org/0000-0002-6813-6276
https://orcid.org/0000-0003-1961-7281
https://orcid.org/0000-0002-6463-362X
https://orcid.org/0000-0002-9006-4619
https://orcid.org/0000-0003-1425-926X
https://orcid.org/0000-0003-2799-6883
https://orcid.org/0000-0002-1096-3236
Assunto:: Peptídeos antimicrobianos
Citocinas
Leishmaniose
Stress oxidativo
Issue Date: 6-Jul-2020
Publisher: MDPI
Citation: KÜCKELHAUS, Selma A. S. et al. Phylloseptin-1 is leishmanicidal for amastigotes of Leishmania amazonensis inside infected macrophages. International Journal of Environmental Research and Public Health, v. 17, n. 13, 4856, 2020. DOI:10.3390/ijerph17134856. Disponível em: https://www.mdpi.com/1660-4601/17/13/4856/html. Acesso em: 19 out. 2020.
Abstract: Leishmania protozoans are the causal agents of neglected diseases that represent an important public health issue worldwide. The growing occurrence of drug-resistant strains of Leishmania and severe side effects of available treatments represent an important challenge for the leishmaniases treatment. We have previously reported the leishmanicidal activity of phylloseptin-1 (PSN-1), a peptide found in the skin secretion of Phyllomedusa azurea (=Pithecopus azureus), against Leishmania amazonensis promastigotes. However, its impact on the amastigote form of L. amazonensis and its impact on infected macrophages are unknown. In this work, we evaluated the effects of PSN-1 on amastigotes of L. amazonensis inside macrophages infected in vitro. We assessed the production of hydrogen peroxide and nitric oxide, as well as the levels of inflammatory and immunomodulatory markers (TGF-β, TNF-α and IL-12), in infected and non-infected macrophages treated with PSN-1. Treatment with PSN-1 decreased the number of infected cells and the number of ingested amastigotes per cell when compared with the untreated cells. At 32 µM (64 µg/mL), PSN-1 reduced hydrogen peroxide levels in both infected and uninfected macrophages, whereas it had little effect on NO production or TGF-β release. The effect of PSN-1 on IL-12 and TNF-α secretion depended on its concentration, but, in general, their levels tended to increase as PSN-1 concentration increased. Further in vitro and in vivo studies are needed to clarify the mechanisms of action of PSN-1 and its interaction with the immune system aiming to develop pharmacological applications.
Licença:: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI: https://doi.org/10.3390/ijerph17134856
Appears in Collections:FMD - Artigos publicados em periódicos e preprints

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