Título:	Marcadores fenotípicos celulares de cães com Leishmaniose Visceral
Autor(es):	Lopes, Giulia da Silva
Orientador(es):	Favali, Cecília Beatriz Fiuza
Coorientador(es):	Mendonça, Lívia Ribeiro
Assunto:	Leishmaniose visceral canina Leucócitos caninos Moléculas de superfície
Data de publicação:	1-Jul-2020
Data de defesa:	13-Fev-2020
Referência:	LOPES, Giulia da Silva. Marcadores fenotípicos celulares de cães com Leishmaniose Visceral. 2020. 160 f., il. Dissertação (Mestrado em Medicina Tropical)—Universidade de Brasília, Brasília, 2020.
Abstract:	Leishmaniasis is a vector-borne zoonosis, belonging to the group of neglected tropical diseases. Visceral Leishmaniasis (VL) is considered the most dangerous form of the disease, presenting a risk of death when left untreated. VL affects humans and other mammal species, being the infected dogs considered the main domestic reservoir of the parasite. In Brazil, Canine Visceral Leishmaniasis (LVC) is generally caused by the species L. infantum, and manifests a broad clinical spectrum, from asymptomatic to symptomatic form of the disease, with intense involvement of internal organs. For the clinical course of the disease, the cellular immune response has an important role, however, there are few studies characterizing in detail the expression of surface molecules in canine leukocytes, mainly differentiating symptomatic from asymptomatic. Therefore, the aim of this study was to characterize the percentage and phenotype of monocytes and lymphocytes of dogs with LVC, symptomatic and asymptomatic. In this study, 15 dogs from the Distrito Federal (Brazil), naturally infected, were distributed in three groups: uninfected dogs (NI), asymptomatic infected dogs (IAS) and symptomatic infected dogs (IS). Cellular phenotypes of monocytes (CD14 + MHC-II +) T lymphocytes (CD3 +, CD4 +, CD25 +, CD8 +), B lymphocytes (CD19 + CD25 +) and the induction of cell death by the parasite in PBMCs were analyzed, in an Ex vivo context. Additionally, the analysis In vitro was performed using antigens from L. infantum, soluble and recombinant (ADF/Cofilina and RAB1-GTPase), as treatments for the cells mentioned above. The results indicate a lower frequency of circulating monocytes (CD14 + MHC-II +), as well as low expression of CD44, in the IS group, suggesting that the function of antigen presentation in monocyte was compromised. Also, the higher expression of CD4 in lymphocytes of IS dogs compared to IAS dogs is a result that should be better studied to understand its impact on the cellular immune response of IS. In the evaluation of cell death, its modulation by the parasite was demonstrated in dogs with LVC, this strategy was used as to evade the immune system. Some biomarkers based on the expression of molecules and percentage of monocytes and lymphocytes, described in this study, can be used to identify the host's susceptibility or resistance profile. The recombinant proteins used in the study need to be better understood to identify their immunogenic potential and possible use in diagnostic methods.
Unidade Acadêmica:	Faculdade de Medicina (FM)
Informações adicionais:	Dissertação (mestrado)—Universidade de Brasília, Faculdade de Medicina, Pós-graduação em Medicina Tropical, 2020.
Programa de pós-graduação:	Programa de Pós-Graduação em Medicina Tropical
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoas de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Apoio e Pesquisa do Distrito Federal (FAP-DF)
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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