Título:	Efeitos da eletroeletroestimulação na morfometria tecidual da pelve e períneo de ratas Wistar
Autor(es):	Sousa, Hugo Alves de
Orientador(es):	Kuckelhaus, Selma Aparecida Souza
Coorientador(es):	Godoy, José Roberto Pimenta de
Assunto:	Eletroestimulação Força muscular Manometria Órgãos pélvicos Assoalho pélvico
Data de publicação:	12-Fev-2020
Data de defesa:	8-Jun-2017
Referência:	SOUSA, Hugo Alves de. Efeitos da eletroeletroestimulação na morfometria tecidual da pelve e períneo de ratas Wistar. 2017. xviii, 102 f., il. Tese (Doutorado em Ciências Médicas)—Universidade de Brasília, Brasília, 2017.
Abstract:	Electrical stimulation (EE) is widely used in several disorders of organic systems, especially in clinical hypotonia, incontinence and denervation. Pelvic and perineal tissues may present dysfunctions due to aging and mechanism of delivery, manifesting in urinary and fecal incontinence, as well as pelvic organ prolapses. In these cases, EE promotes functional improvement; however, its effects on tissues are unknown. The murine model has been proposed to elucidate the physiology and the morphological aspects of the continence and how EE improves the function of the pelvic and perineal organs. Considering that, it is fundamental to elucidate the effects and mechanisms of the EE, our objective was to produce a probe to EE and a manometer, both adapted to the vaginal and anorectal dimensions of nulliparous Wistar rats, as well as to evaluate the effects of EE on the tissues of the pelvis and perineum. Thus, the study was performed with groups of five animals/group. In the pared study was evaluate the intra-anal pressure, in the resting and in the intravaginal EE, while, the unpaired study consisting of three groups (Control, intravaginal EE and intra-anal EE) it was determined the effect of 30 EE sessions, during three consecutive weeks (frequency = 50 Hz; pulse width = 700 μs; intensity = 2 to 4 mA. Afterwards, the animals were euthanized and the pelvic segments were dissected and processed according to standard protocol for optical microscopy. The results showed that: 1) the probe presented adequate dimensions for the rats, being able to induce the effective EE of the pelvic and perineal tissues; 2) the manometer allowed adequate recording of pressure data; 3) intra-anal pressure increased with the electric current indicating muscle contraction; 4) Both EE routes (intravaginal or intra-anal) increased the widths of the mucosa, submucosa and muscular layers of the urethra, vagina, rectum and anus; however, the intravaginal route reduced the width of the mucosa of the vagina; 5) both routs increased the diameter of the skeletal fibers and reduced their number in the pelvic floor muscles (MAPS) and in the external anal sphincter (EAE), increased the number of myonuclei of the skeletal fibers of the MAPs, increased the number of smooth fibers in the urethral, vagina, rectum and in the internal anal sphincter, increased the width of the parietal and visceral endopelvic fascia; 6) intravaginal EE increased the width of mucosal and submucosal, as well, the number of smooth muscle and the diameter of the striated fibers of the external urethral sphincter, when compared to the intra-anal route; 7) the intra-anal route was more effective than the intravaginal route to increase the width of the mucosa and the total muscle fibers of the vagina; 8) there were no differences between intravaginal and intra-anal EE routes in the rectum tissues. Our results have demonstrated the efficacy of the EE probe in transmitting the electrical signal to stimulate the muscle fibers and to activate the quiescent satellite cells, resulting in cellular hypertrophy, in addition to activating fibroblasts resulting in the widest width of the endopelvic fascia. Thus, our results point to the effectiveness of EE in the functional gain mediated by the structural improvement of the tissues of the pelvic organs.
Unidade Acadêmica:	Faculdade de Medicina (FM)
Informações adicionais:	Tese (doutorado)—Universidade de Brasília, Faculdade de Ciências Médicas, Programa de Pós-Graduação em Ciências Médicas, 2017.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências Médicas
Licença:	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
DOI:	http://dx.doi.org/10.26512/2017.06.T.36895
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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